Protein Info for Pf6N2E2_2332 in Pseudomonas fluorescens FW300-N2E2

Annotation: DNA ligase (EC 6.5.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 785 PF01653: DNA_ligase_aden" amino acids 4 to 324 (321 residues), 427.8 bits, see alignment E=7.8e-132 TIGR00575: DNA ligase, NAD-dependent" amino acids 11 to 607 (597 residues), 739.2 bits, see alignment E=2.1e-226 PF03120: DNA_ligase_OB" amino acids 329 to 405 (77 residues), 117.8 bits, see alignment E=4.7e-38 PF14520: HHH_5" amino acids 466 to 509 (44 residues), 17.5 bits, see alignment 1.4e-06 PF12826: HHH_2" amino acids 529 to 592 (64 residues), 71.4 bits, see alignment E=1.7e-23 amino acids 624 to 679 (56 residues), 29.3 bits, see alignment 2.3e-10 PF00533: BRCT" amino acids 707 to 775 (69 residues), 41.3 bits, see alignment E=4.4e-14

Best Hits

Swiss-Prot: 89% identical to DNLJ_PSEPF: DNA ligase (ligA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 98% identity to pba:PSEBR_a1707)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.2

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXF9 at UniProt or InterPro

Protein Sequence (785 amino acids)

>Pf6N2E2_2332 DNA ligase (EC 6.5.1.2) (Pseudomonas fluorescens FW300-N2E2)
MTAVETRIQQLRTELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEEQHPELVTSDSPTQ
RVGSMALSAFSQVRHEIPMLSLGNAFDEATMREFDRRVTEGLDLPMGDLLGGGAAVEYSC
EPKLDGLAVSLLYQDGLLVRGATRGDGTTGEDISVNVRTVRNIPLKLQGSGWPPLLEVRG
EVYMSKAGFERLNATQLEAGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGIGQVTT
DIADTHIGNLKQLKAWGLPISRELKLAHGIDECLDYYRDIGERRNALPYEIDGVVFKVNS
IASQRELGFRAREPRWAIAHKFPASEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVTV
ANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVITERRPEHARPVAIPQQCPVCGS
HVERTQLIKRSKGRETISEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIEGLGEKSVEQ
LVDEGLVGSPADLYALTFEQVVDLEGFAELSSKNLLAAIVDSKKPSLARFIYALGIPDVG
EETAKVLARSLGSLERVQAALPQVLTYLPDVGLEVAHEIHSFFEDPHNRQVIKDLLRHGL
EIQDQGELGAEFSASTTLGGFLDKLDIPSVGPGGAQKLADKFGSLEAVMNADWLDMRQAL
PEKQANAVREFFANASNRQQAEAAERQLRDFGMHWQSEKKVVEGLPEAGHTWVLTGSLEL
MSRDVAKDKLESLGAKVAGSVSVKTHCVVAGPGAGSKLTKANELGLKVLDEEAFVAFLSK
HGITV