Protein Info for Pf6N2E2_2267 in Pseudomonas fluorescens FW300-N2E2

Annotation: Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 154 to 174 (21 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 5 to 447 (443 residues), 502.5 bits, see alignment E=6.2e-155 PF21085: CusS" amino acids 6 to 148 (143 residues), 26.5 bits, see alignment E=1.6e-09 PF00672: HAMP" amino acids 172 to 224 (53 residues), 39.6 bits, see alignment 1.3e-13 PF00512: HisKA" amino acids 230 to 295 (66 residues), 58.5 bits, see alignment E=1.4e-19 PF02518: HATPase_c" amino acids 338 to 449 (112 residues), 90.6 bits, see alignment E=2.3e-29

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 97% identity to pba:PSEBR_a1769)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z7H9 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Pf6N2E2_2267 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) (Pseudomonas fluorescens FW300-N2E2)
VRGRWSLSSRLALLFAACTAVVSLFAGVLFSRGSEAHFIELDQQLLEGKLIGLRRALQDL
DAEQTERRLEDELSRQADLALRIKGSDGVRWYDSSIRIPAQLPEQPGLSTLSDADNDYRV
LNAPLYPNRANSPQLTLLLDITHHQHFLQRMQRLIWLTVGLSALATALLGAWAARRALRP
LRRMGAIAGSVSARSLNARLPEEQMPAELAELAHSINAMLGRLDDAFQRLSAFSADIAHE
LRTPLSNLLTHTQVTLTRERPLEDYREALHSNLEELQWMAQLVNDMLYLAKADHGLLALN
REPLQLAEEVDVLLDFFAPLAEDTNVRLSREGDGRIEGDRNMLRRALSNLLDNALRFTPA
DGEVRVHIVDATQGVSVSVENSGEGIAADLLPRLFDRFYRADPARQEGSSEHAGLGLAIT
RSIIRAHGGQIRCESADGWTRFLIELPKTSQP