Protein Info for Pf6N2E2_2248 in Pseudomonas fluorescens FW300-N2E2

Annotation: Aspartate racemase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF01177: Asp_Glu_race" amino acids 49 to 238 (190 residues), 54.2 bits, see alignment E=8.7e-19 amino acids 256 to 478 (223 residues), 142.2 bits, see alignment E=1.1e-45 TIGR00035: aspartate racemase" amino acids 253 to 485 (233 residues), 179.3 bits, see alignment E=4.6e-57

Best Hits

KEGG orthology group: K01779, aspartate racemase [EC: 5.1.1.13] (inferred from 96% identity to pba:PSEBR_a1787)

Predicted SEED Role

"Aspartate racemase"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZ32 at UniProt or InterPro

Protein Sequence (504 amino acids)

>Pf6N2E2_2248 Aspartate racemase (Pseudomonas fluorescens FW300-N2E2)
MKAKAKSSHKASVVRKLGIVGGLGSLAGGDLFFKLVKSRAVLEDQGRYHFLFEQHPFKDV
LLPLDQGASMTSRKFYVFQVCKSFEDSGFDAVMLPCFASHTFRAEIEEELGIPVLDMMAA
LTRHVSHTVPPQATLGVIASDFVRHCGLFERYFGKDFRIVYPDADAQVGLMEAMYGLNGI
KDGHLEGVPLEAVYRACLSLQAQGATVMLPGMTELSLVCADLQRRGITVLDINEIYADFA
TLDQQPRRPPFKLGIVGGVGPAATVDFMAKVVANTPAGKDQDHIKMVVEQNPQIPDRTAS
LLRDETDPTMAMYATCKRLESAGANAIAIPCNTAHAFVERIQAHLRVPIVNMLSETVEWI
ARTYGSGKAIGLLATSGTVQSQVYHQAARHAGLQVLTPGVDYQAMVMDAIYGPRGIKAGF
TQGLCKEQLLLAAEHLCELGAGVLILGCTELPLVLAHCDAFDINGHRVALVDPTMILALK
CVTLAQRADKPMSVLKTSKDVAAT