Protein Info for Pf6N2E2_2222 in Pseudomonas fluorescens FW300-N2E2

Annotation: Murein-DD-endopeptidase (EC 3.4.99.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 transmembrane" amino acids 16 to 36 (21 residues), see Phobius details PF00768: Peptidase_S11" amino acids 49 to 276 (228 residues), 245.5 bits, see alignment E=5.6e-77 PF13354: Beta-lactamase2" amino acids 59 to 202 (144 residues), 41.9 bits, see alignment E=8.1e-15

Best Hits

Swiss-Prot: 66% identical to PBP7_SHIFL: D-alanyl-D-alanine endopeptidase (pbpG) from Shigella flexneri

KEGG orthology group: K07262, D-alanyl-D-alanine endopeptidase (penicillin-binding protein 7) [EC: 3.4.21.-] (inferred from 97% identity to pba:PSEBR_a1811)

MetaCyc: 66% identical to peptidoglycan DD-endopeptidase PbpG (Escherichia coli K-12 substr. MG1655)
3.4.-.-

Predicted SEED Role

"Murein-DD-endopeptidase (EC 3.4.99.-)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.99.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-, 3.4.99.-

Use Curated BLAST to search for 3.4.21.- or 3.4.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z754 at UniProt or InterPro

Protein Sequence (325 amino acids)

>Pf6N2E2_2222 Murein-DD-endopeptidase (EC 3.4.99.-) (Pseudomonas fluorescens FW300-N2E2)
LLNGTFNSIEPTRVKIRLSIISLFIAFTGSFITQNVAAQETIAAPRDTAKLQVASGSAML
LDLQTDKVVYSSNPDVVVPIASVTKLMTGLIVLDAKQNLDEYISITIANTPEMKGVFSRV
KLNSELSRRDVLLIALMSSENRAAASLAHHYPGGYAAFIAAMNAKAKALGMTSTHYVEPT
GLSIHNVSTARDLTKLLIAARKYPLLSELSTTKEKTVTFRKPVYSLGFRNTDHLVHKANW
DIQLTKTGFTNQAGHCLVLVTKMGNRPMALVILDAFGKYTHFADASRIRSWVETGKSAAV
PAVALQYKAAKNLKSRQSGVVEASK