Protein Info for Pf6N2E2_2192 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4)
Rationale: Specifically important for utilizing L-Leucine. Automated validation from mutant phenotype: the predicted function (6.4.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 PF01039: Carboxyl_trans" amino acids 48 to 525 (478 residues), 536.2 bits, see alignment E=7.4e-165 PF03255: ACCA" amino acids 90 to 207 (118 residues), 30.2 bits, see alignment E=2.7e-11

Best Hits

Swiss-Prot: 68% identical to MCCB_MOUSE: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (Mccc2) from Mus musculus

KEGG orthology group: K01969, 3-methylcrotonyl-CoA carboxylase beta subunit [EC: 6.4.1.4] (inferred from 100% identity to pba:PSEBR_a1844)

MetaCyc: 86% identical to methylcrotonyl-CoA carboxylase beta-subunit (Pseudomonas aeruginosa PAO1)
Methylcrotonoyl-CoA carboxylase. [EC: 6.4.1.4]

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z6X2 at UniProt or InterPro

Protein Sequence (535 amino acids)

>Pf6N2E2_2192 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) (Pseudomonas fluorescens FW300-N2E2)
MATLHTQLNPRSPEFIANRDAMLGHVEALRTLLAQIRQGGGPKAQERHTSRGKLLPRERI
NRLLDPGSPFLEISPLAAHEVYGEDVPAAGVIAGIGRVEGVECMIVANDATVKGGSYYPL
TVKKHLRAQTIAQQNRLPCIYLVDSGGANLPRQDEVFPDREHFGRIFFNQANMSAQGIPQ
IAVVMGSCTAGGAYVPAMADEAIMVRQQATIFLAGPPLVKAATGEVVSAEDLGGADVHCK
ISGVADHYADSDEHALALARRSVANLNWRKQGELQHRLPIAPLYSGEELYGVVSADAKQP
FDVREVIARLVDGSVFDEFKALFGTTLVCGFAHLHGYPIAILANNGILFAEAAQKGAHFI
ELACQRGIPLLFLQNITGFMVGQKYEAGGIAKHGAKLVTAVACAKVPKFTVIIGGSFGAG
NYGMCGRAYDPRFLWMWPNARIGVMGAEQAAGVLVQVKREQAERSGHPFSAEQEAEIKQP
ILDQYEEQGHPYYSSARLWDDGVIDPAQTRDVLGLALSASLNAPIEPSRFGVFRM