Protein Info for Pf6N2E2_2169 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cysteinyl-tRNA synthetase (EC 6.1.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 TIGR00435: cysteine--tRNA ligase" amino acids 1 to 454 (454 residues), 430.2 bits, see alignment E=6e-133 PF01406: tRNA-synt_1e" amino acids 16 to 324 (309 residues), 316.9 bits, see alignment E=1.6e-98

Best Hits

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXS7 at UniProt or InterPro

Protein Sequence (456 amino acids)

>Pf6N2E2_2169 Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Pseudomonas fluorescens FW300-N2E2)
MELFNTLGRAVVPFIPIVENHVGLYACGPTVYHYAHIGNMRTYIFEDVLAKTLRHRGLVV
KHVMNITDVGHLQSDADAGHDKLMVGAARENKTPWQVAQFYEEAFFRHCGLLNITRPDVV
CRATQHIPEIITLIERLLEKGVAYESAGNVYFSVEKFPAYHELGQLVLDEQRATERVSHD
PLKRSQADFALWFSTSKFPGQIMKWESPWGLGFPGWHIECSAMASKYLGERVDIHCGGID
HINVHHSNEIAQSECCFGHRWVNYWFHCEFLNVDTEKMSKSSGEFLTVDSLVDKGYDPCA
FRLLVLGSHYRSALAFSWEALDDVATALQKARRRISIALDNSSSPGEAFSAMHAASYRRF
FAALEKDLHTPSAMAEFWWVIKTNELTEPERIDLLRSFNAVLDIGLFDTQATRLTSEQSD
LIAARNVARNERNWAESDRIRDLLLAQGISLSDLKA