Protein Info for Pf6N2E2_2052 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): ABC transporter for D-Glucosamine Hydrochloride, permease component 2
Rationale: Specific phenotypes on D-Glucosamine Hydrochloride; D-Glucosamine Hydrochloride. ko:K02029 : polar amino acid transport system permease protein
Original annotation: amino acid ABC transporter, permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 220 transmembrane" amino acids 20 to 47 (28 residues), see Phobius details amino acids 58 to 80 (23 residues), see Phobius details amino acids 92 to 112 (21 residues), see Phobius details amino acids 155 to 156 (2 residues), see Phobius details amino acids 158 to 170 (13 residues), see Phobius details amino acids 190 to 212 (23 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 15 to 111 (97 residues), 96 bits, see alignment E=7.8e-32 PF00528: BPD_transp_1" amino acids 36 to 216 (181 residues), 77.4 bits, see alignment E=6.2e-26

Best Hits

Swiss-Prot: 40% identical to YECS_ECOL6: L-cystine transport system permease protein YecS (yecS) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02029, polar amino acid transport system permease protein (inferred from 99% identity to pba:PSEBR_a1959)

MetaCyc: 40% identical to cystine ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-290 [EC: 7.4.2.12]; 7.4.2.12 [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]

Predicted SEED Role

"amino acid ABC transporter, permease protein"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWH7 at UniProt or InterPro

Protein Sequence (220 amino acids)

>Pf6N2E2_2052 ABC transporter for D-Glucosamine Hydrochloride, permease component 2 (Pseudomonas fluorescens FW300-N2E2)
MNYQLNFAAVWRDFDTLLAGLGLGLSLALVSIAIGCVIGLAMAFALLSKHRVLRVLASVY
VTVIRNTPILVLILLIYFALPSLGIRLDKLPSFVITLSLYAGAYLTEVFRGGLLSIHKGQ
REAGLAIGLGEWQVKAYVTVPVMLRNVLPALSNNFISLFKDTSLAAAIAVPELTYYARKI
NVESYRVIETWLVTTALYVAACYLIAMLLRYFEQRLAIRR