Protein Info for Pf6N2E2_2043 in Pseudomonas fluorescens FW300-N2E2

Annotation: Twitching motility protein PilT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 TIGR01420: twitching motility protein" amino acids 27 to 366 (340 residues), 432.1 bits, see alignment E=8e-134 PF00437: T2SSE" amino acids 81 to 295 (215 residues), 136.7 bits, see alignment E=4.4e-44

Best Hits

Swiss-Prot: 37% identical to PILT_NEIGO: Twitching motility protein (pilT) from Neisseria gonorrhoeae

KEGG orthology group: K02670, twitching motility protein PilU (inferred from 98% identity to pba:PSEBR_a1969)

Predicted SEED Role

"Twitching motility protein PilT" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GMN8 at UniProt or InterPro

Protein Sequence (395 amino acids)

>Pf6N2E2_2043 Twitching motility protein PilT (Pseudomonas fluorescens FW300-N2E2)
VRAGLKGFMFGSTCFIDVSRKFQSMEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKP
LGNEAFKVGDIARLAGSLMDAEQRLEFDRELEMNLAISLAGVGRFRVNIFKQRNDVSMVI
RNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHII
TIEDPVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALA
FADTGHLVISTLHAHNANQALDRVINFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQR
RAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDHALFNLVVEGAIDEEEALK
NADSANNLRLRLKLHTESGAVPPADPAAGEWGLMD