Protein Info for Pf6N2E2_1977 in Pseudomonas fluorescens FW300-N2E2

Annotation: DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 TIGR01056: DNA topoisomerase III" amino acids 24 to 469 (446 residues), 537.6 bits, see alignment E=2.7e-165 PF01751: Toprim" amino acids 26 to 152 (127 residues), 55.6 bits, see alignment E=5.1e-19 PF01131: Topoisom_bac" amino acids 171 to 469 (299 residues), 273.3 bits, see alignment E=3.9e-85

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (516 amino acids)

>Pf6N2E2_1977 DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster (Pseudomonas fluorescens FW300-N2E2)
LRVARTVSPHASSCPLNCFAGHDMRLFLCEKPSQGKDIARVLGARQRGSGCFKGVGISVT
WCIGHLVEAAPPETYDERFKQWSLDHLPILPISWRVRVKANTAAQYKIVKQLLAQTSELV
IATDADREGEMIAREVLELCQYRGSIQRLWLSALNDASIRKALSQLRQAEETQSLYHSAL
ARSRADWLIGMNMSRLFTLLGRQAGLNGVLSVGRVQTPTLRLVVDRDREIERFVPRPYWV
IQVKLEVLGQAFTAQWAAPDSATDEAGRCIRQAIALQAQQSFKATGVAQVLEVETQRTRE
APPLPFDLNTLQEVCSRRLGYGVQETLDIAQSLYETHKATTYPRTDSGYLPESMLLEAEA
VFAALIKSDPGVGPLVQSVDFTLRSRAWNDSQVSAHHGIIPTLEPFDRLALSERECAVYE
LIRARYLAQFLPPHEYDRTAVQLTCGNHVLKAHGKHVVESGWHRVLGSECPPQRRRSVAT
QPNATRPARGCLLQRGGRGAKVPQDSAPPKLIPRVS