Protein Info for Pf6N2E2_1949 in Pseudomonas fluorescens FW300-N2E2
Annotation: RND efflux system, inner membrane transporter CmeB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to MEPB_PSEPU: Multidrug/solvent efflux pump membrane transporter MepB (mepB) from Pseudomonas putida
KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 63% identity to abo:ABO_0964)MetaCyc: 64% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592
Predicted SEED Role
"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZX99 at UniProt or InterPro
Protein Sequence (1051 amino acids)
>Pf6N2E2_1949 RND efflux system, inner membrane transporter CmeB (Pseudomonas fluorescens FW300-N2E2) MSKYFIDRPIFAWVLALIIMLVGALSILNLPINQYPDIAPPAVAIQVSYPGASAQTVQDT VVQVIEQQMNGIDNLRYISSESNADGSMRIIVTFNQGTNPDIAQVQVQNKLNLATPLLPR EVQQQGLRVTKFQQNFMMFIGLVSTDGKHTKEDLSNYIVSNIQDPISRTSGVGDFQIWGT QYAMRVWLDPAKLNQFQLTPVDVTEAIEAQNVQVASGTIGGLPARKGTVFTATIIGKTRL QTSEQFGDILLKVNSDGSQVRLSDVADISLGGENYSINAQANGKPASGIAIRLATGANAL DTARAIRATVDRLKPFFPAGIEAVYPYDTTPVVTESISGVMHTLGEAVVLVFLVMLLFLQ NLRATIITTLTVPVVLLGTFGVLAAAGFSINTLTMFGMILAIGLLVDDAIVVVENVERVM AEEHLSPREATRKSMGQIQGALVGIALVLSAVLLPMAFFGGSTGVIYRQFSITIVSAMAL SVFVALVFTPALCATLLKPIDPTKHGQPKRGFFGWFNRRFDACVNGYEQGVGQIIRHRVP GFMVYLVIVAGTIWMFARIPTSFLPDEDQGLIFTQVQTPANSSAETTQKVLDRMSQYLLD KEHGEGKAVATVFTVNGFNFAGRGQNSGIAFVNLKPWDERDSDNTVFKIAQRAQQEFLKY RDALVYAVVPPSVLELGNATGFDFYLQDQDSVGHQKLMEARGKFLELAAQSPVLAGVRPN GLADEPQYHLTIDDERARAMGISLADINTTLSVAYGSSYVNDFVDRGRVKRVYVQGQPDS RSTAEDLSKWFVRNNQGQMVPFSAFASAEWIFGSPKLSRYNGVPAEQILGTPAPGYSSGQ AMAEVERIAEQLPPGVGISFTGLSYEERLSGSQAPALYALSILVVFLCLAALYESWSIPI AVILVVPLGVIGALMATALRGLSNDVFFQVGLLVTVGLAAKNAILIVEFAKELNEKGQPL LEATVEACRMRLRPIVMTSLAFILGVLPLTISSGAGSGSQHAIGTGVIGGMITATVLAIF WVPLFFVSISTLFKGSRKKRDETAMREEAGQ