Protein Info for Pf6N2E2_1940 in Pseudomonas fluorescens FW300-N2E2

Annotation: TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR02485: precorrin 3B synthase CobZ" amino acids 2 to 466 (465 residues), 670.8 bits, see alignment E=4e-206 PF01266: DAO" amino acids 3 to 119 (117 residues), 28 bits, see alignment E=4.9e-10 PF00890: FAD_binding_2" amino acids 3 to 441 (439 residues), 112.1 bits, see alignment E=1.2e-35 PF12831: FAD_oxidored" amino acids 3 to 179 (177 residues), 36.5 bits, see alignment E=1.1e-12

Best Hits

KEGG orthology group: K13796, tricarballylate dehydrogenase (inferred from 99% identity to pba:PSEBR_a2045)

MetaCyc: 75% identical to propane-1,2,3-tricarboxylate dehydrogenase (Salmonella enterica enterica serovar Typhimurium str. LT2)
1.3.8.M1 [EC: 1.3.8.M1]

Predicted SEED Role

"TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate" in subsystem Tricarballylate Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.M1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZX91 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Pf6N2E2_1940 TcuA: flavoprotein used to oxidize tricarballylate to cis-aconitate (Pseudomonas fluorescens FW300-N2E2)
MVDVLVIGGGNAALCAALMAREAGASVMLLEGSPKVWRGGNSQHTRNLRCMHDAPQDVLV
DAYPEEEYWQDLLKVTGGLTNEKLARIAIRASSSCRDWMRSHGVHFQPPLSGALHVARTN
AFFMGGGKALVNAYFRSAERLGVKIRYDAQVTDIELENGAFVAAHLAERMVGGQRFPAER
IEARACVLAAGGFESNREWLREAWGQNERGEWPSDNFLIRGTRFNTGVLLRRMLDLGADA
VGDPTQAHMVAIDARAPLYDGGICTRIDCVSLGVVVNRDGERFYDEGEDFWPKRYAIWGR
LVAGQPNQQAYSIIDQQALGRFMPPVFPGTTARTLPELARLLNLPQTQFVKTIEDYNNAC
HVGTFDHTTLDDCHTEGLIPAKTHWARPLTKPPYYAYPLKPGVTFTYLGLATDETAAVHF
DGKASPNLFVAGEMMAGNVLGKGYTAGIGMAIGTAFGRIAGVQAALCAGFGLSPVKSELQ
HAAV