Protein Info for Pf6N2E2_1930 in Pseudomonas fluorescens FW300-N2E2

Annotation: L-Proline/Glycine betaine transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 transmembrane" amino acids 18 to 42 (25 residues), see Phobius details amino acids 50 to 75 (26 residues), see Phobius details amino acids 84 to 102 (19 residues), see Phobius details amino acids 108 to 128 (21 residues), see Phobius details amino acids 149 to 172 (24 residues), see Phobius details amino acids 184 to 203 (20 residues), see Phobius details amino acids 244 to 267 (24 residues), see Phobius details amino acids 279 to 299 (21 residues), see Phobius details amino acids 308 to 328 (21 residues), see Phobius details amino acids 340 to 361 (22 residues), see Phobius details amino acids 373 to 394 (22 residues), see Phobius details amino acids 402 to 420 (19 residues), see Phobius details PF07690: MFS_1" amino acids 17 to 281 (265 residues), 68 bits, see alignment E=7.6e-23 amino acids 283 to 424 (142 residues), 41.7 bits, see alignment E=7.2e-15 PF00083: Sugar_tr" amino acids 17 to 216 (200 residues), 77.1 bits, see alignment E=1.4e-25 amino acids 254 to 428 (175 residues), 47.9 bits, see alignment E=9.7e-17

Best Hits

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 100% identity to pba:PSEBR_a2056)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9X6H3 at UniProt or InterPro

Protein Sequence (456 amino acids)

>Pf6N2E2_1930 L-Proline/Glycine betaine transporter ProP (Pseudomonas fluorescens FW300-N2E2)
MTKTKTTSSRRSLAAGAIGNFGEIYDFAVFGFSIPILSVHFFPGSDRTAALLSTFAVYAV
AFVARPLGGLMFGYLADRLGRIRVMAMTVWLMALGTAIIGLLPTYATIGIAAPLLLLLCR
IAQGLALGGETTGSTSYIVESAPENRRGYWLGFTLIFSHLPNAVVAGLVVALQLGAGDQA
YSDWAWRIPFLLGGIIGVVGFWLRRNIDEPEEYKQARQASKASKIKKNPLIAAIRCGGLR
GMLHVFMVQPVFSVGAYLLLGFMYTFLIEVGKLDSTSALISNAIAVIVLSALLPLGGLLS
DRFGRKRVLTFGAAWIALSAYPAMYLAASGSFASAVAGQTLLAAGLGIYGAASFVAAAEF
FPTSFRATGHAISYQTSVAMFGGTCPLIAAYLSQAFGSPLAPAFYVTLIAVLCLITTQFV
PETRGVNLRTSVGNKTSNKASELPQPQKAMRQELST