Protein Info for Pf6N2E2_188 in Pseudomonas fluorescens FW300-N2E2

Annotation: Formamidase (EC 3.5.1.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03069: FmdA_AmdA
  • amino acids 7 to 384 (378 residues), 598.8 bits, E=1.9e-184

Best Hits

Swiss-Prot: 70% identical to FMDA_METME: Formamidase (fmdA) from Methylophilus methylotrophus

KEGG orthology group: K01455, formamidase [EC: 3.5.1.49] (inferred from 100% identity to pba:PSEBR_a3686)

MetaCyc: 55% identical to formamidase subunit (Paracoccidioides brasiliensis)
Formamidase. [EC: 3.5.1.49]

Predicted SEED Role

"Formamidase (EC 3.5.1.49)" (EC 3.5.1.49)

MetaCyc Pathways

• cyanide detoxification II (1/2 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.49

Sequence Analysis Tools

See A0A165YMH0 at UniProt or InterPro

Protein Sequence (409 amino acids)

>Pf6N2E2_188 Formamidase (EC 3.5.1.49) (Pseudomonas fluorescens FW300-N2E2)
MTDTLIKVDLNQPATENEQIHNRWHPDIPMACWVKPGDDFILETYDWTAGAIKNNDDASD
VRDVDLSTVHYLSGPVGVHGAEPGDLLVVDLLDIGAKAESMWGFNGFFSRQNGGGFLTDH
FPSAQKAIWDFKGMFTSSRHIPGVNFAGLIHPGLIGCLPDPVMLGDWNRREQELIDTHPT
RVPPLANAPLAATAHMGKLKGQARDDAAATGARTVPPREHGGNCDIKDLSRGSKIFFPVY
VKGAGLSVGDLHFSQGDGEITFCGAIEMAGWVHMKVELIKGGMAKYGIKNPVFKPSPIIP
TYQNYLIFEGISVDEAGKQHYLDVNIAYRQACLNAINYLTKFGYSPAQGYALLGSAPVQG
HISGVVDIPNACATLWLPTEIFEFDINPNANGPIKHLDGSIDLPIAPDL