Protein Info for Pf6N2E2_1838 in Pseudomonas fluorescens FW300-N2E2

Annotation: 6-hexanolactone hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 PF20434: BD-FAE" amino acids 68 to 155 (88 residues), 42.6 bits, see alignment E=1e-14 PF10340: Say1_Mug180" amino acids 69 to 186 (118 residues), 44.2 bits, see alignment E=2.5e-15 PF07859: Abhydrolase_3" amino acids 72 to 274 (203 residues), 190.8 bits, see alignment E=5.5e-60

Best Hits

KEGG orthology group: None (inferred from 48% identity to bbr:BB4484)

MetaCyc: 44% identical to N-acetyl-phophinothricyl-L-alanyl-L-leucine acetyl hydrolase (Kitasatospora phosalacinea)
3.1.1.-

Predicted SEED Role

No annotation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZY76 at UniProt or InterPro

Protein Sequence (301 amino acids)

>Pf6N2E2_1838 6-hexanolactone hydrolase (Pseudomonas fluorescens FW300-N2E2)
MRAQSEAVERVIARIQTVYGRWRRHTPIETLRRDWDDLFWADKLTCETQDASANGVPVRW
IKTPGVAEQQVLIYLHGGGFKMGSLTSHHDLMVRLSVAAGCRILGVDYRLLPEHGFPEPL
LDVLAVYDWLVAQGFEPDQLAIAGDSAGGGLAAAALLALRDKGRPLPAAAVLLSAWTDLS
ASGESYLTRAESDPIHQRPMIVALAAQYLGKDGDPFDPLASPLHGDLTGLPPLLLQVGDR
ETGLDDSTLFAAKARAVGVAVELQVWPQMFHVFQQFAAELAEAREAIACIGAFLRKHWNL
K