Protein Info for Pf6N2E2_1830 in Pseudomonas fluorescens FW300-N2E2

Annotation: Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 154 to 269 (116 residues), 84.2 bits, see alignment E=1.8e-27 PF02770: Acyl-CoA_dh_M" amino acids 274 to 382 (109 residues), 52.4 bits, see alignment E=9.6e-18 PF00441: Acyl-CoA_dh_1" amino acids 398 to 546 (149 residues), 116.7 bits, see alignment E=2.2e-37 PF08028: Acyl-CoA_dh_2" amino acids 419 to 533 (115 residues), 37.7 bits, see alignment E=4.5e-13

Best Hits

KEGG orthology group: K14448, (2S)-methylsuccinyl-CoA dehydrogenase (inferred from 57% identity to gpb:HDN1F_16010)

Predicted SEED Role

"Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZU97 at UniProt or InterPro

Protein Sequence (556 amino acids)

>Pf6N2E2_1830 Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007) (Pseudomonas fluorescens FW300-N2E2)
MLPITATNDLLGLLLQLRGVLEQATSALASRCTKGQQLDADLLDLMQVPTFELAWASAEL
LAAERSLQAIDAGTSAVDRRLILVFAVEAITLVHGRLEAICVELDLADGALHAIAADPKL
RALRRSVLSSVALHDSARLMVERPEQIGQVAMGDELSMIQDQFRRFAADTVAPLAEHIHR
EDLIIPDSLLAALRDMGVFGLSIPERYGGSAPDDQEDPLTMIVVTEALSQASLAAAGSLI
TRPEILSRALLSGGTEAQKQHWLPRLAVGAPLCAIAITEPDYGSDVASLTLRGTPCEGGW
RLNGAKTWCTFAGKAEVLMVVTRTNPDKSLGHRGLSLLLAEKPSYDGHDFDFRQPGGGRL
TGRAIPTIGYRGMHSFDLSFEDFFVPDGNVIGEAQGLGKGFYHTMAGMTGGRMQTAGRAS
GVMRAALLAGLRYATERKVFGAPLLDYPLTGAKLAKMAARYVASRYLTYSVGCMLAQGAG
RMEASLVKLFACRSAELVTRESLQIHGGMGYAEEVAVSRYFVDARVLSIFEGAEETLALK
VIGRSLLEAALKAEVA