Protein Info for Pf6N2E2_1827 in Pseudomonas fluorescens FW300-N2E2

Annotation: Choline-sulfatase (EC 3.1.6.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00884: Sulfatase" amino acids 30 to 440 (411 residues), 184.6 bits, see alignment E=4.2e-58 PF01663: Phosphodiest" amino acids 307 to 363 (57 residues), 24.1 bits, see alignment 3.9e-09 PF16347: SGSH_C" amino acids 399 to 525 (127 residues), 37.7 bits, see alignment E=3.4e-13

Best Hits

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZW04 at UniProt or InterPro

Protein Sequence (559 amino acids)

>Pf6N2E2_1827 Choline-sulfatase (EC 3.1.6.6) (Pseudomonas fluorescens FW300-N2E2)
MKKNLNWLASSALLVVACLAPPAFAASAKPNILLIVADDLGYSDLGAFGGEIDTPNLDEL
SKHSTLLQSLYVAPTCSPTRSMLMSGTDNHLAGVGNMAEMMTPHLTPEQMGKPGYEGYLN
QRVAALPELMKDAGYQTFMVGKWHLGGADGLRPQQRGFDKSYVLMGGGAAHFKQTAPMQI
ASDAPEPVYRENDIKVELPADFYSSTNFTNKLIEYVDAGLGEKKPFFAYAAYTAAHLPSQ
APDAYLQKYRGRYDQGYEVTAARRIEKLKSLGLVDAKATPVPMPKGVKPWSALSVEEKEY
SARTMEAYAAMVDAFDHEIGRLINHLKDKGLYDNTLIVFLSDNGPEGNDWSKDVENDQWI
PKHFDVSLNNIGRPNSFAYEGPAWGQVSAQPFRMFKAYTNEGGVRSASFMRLPFNDNPQR
TDAVVTAMDIMPTILDVAGIKHPGTQYQGKTVEPMKGASALSMLQGKSHAVHDSTYTVGI
ELMGRNAIRKGNWKIVYSFDDGKGTWALYDLATDRGELHDLSSQHPDKLNELLTEWAQYV
RQNNVISTGRDTTYPRRDY