Protein Info for Pf6N2E2_178 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 791 (791 residues), 860.5 bits, see alignment E=6.4e-263 PF01588: tRNA_bind" amino acids 45 to 144 (100 residues), 78.5 bits, see alignment E=8.3e-26 PF03483: B3_4" amino acids 211 to 384 (174 residues), 245.3 bits, see alignment E=7.2e-77 PF03484: B5" amino acids 405 to 471 (67 residues), 75.2 bits, see alignment E=9.8e-25 PF17759: tRNA_synthFbeta" amino acids 474 to 682 (209 residues), 217 bits, see alignment E=5.6e-68 PF03147: FDX-ACB" amino acids 698 to 791 (94 residues), 104.2 bits, see alignment E=1e-33

Best Hits

Swiss-Prot: 93% identical to SYFB_PSEPF: Phenylalanine--tRNA ligase beta subunit (pheT) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 98% identity to pba:PSEBR_a3696)

MetaCyc: 52% identical to phenylalanine--tRNA ligase subunit beta (Escherichia coli K-12 substr. MG1655)
Phenylalanine--tRNA ligase. [EC: 6.1.1.20]; 6.1.1.20 [EC: 6.1.1.20]; 3.1.1.- [EC: 6.1.1.20]

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YM09 at UniProt or InterPro

Protein Sequence (792 amino acids)

>Pf6N2E2_178 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) (Pseudomonas fluorescens FW300-N2E2)
MKFSEQWLRGWVSPQVSRDELVARLSMAGLEVDSVTPAAGDFSGVVVGEVLGTEQHPDAD
KLRVCQVSNGAETFQVVCGAPNVRPGLKIPFAMIGAELPGDFKIKKAKLRGVESNGMLCS
QAELKIGEGNDGLMELPADAPVGQDIREYLGLDDASIEVDLTPNRGDCLSLAGLAREVGA
LYAVPVTRPVVAAVPAVHDEVRAVEVLAPAACPRYLGRVIRNVDLSKPTPLWMVERLRRA
DVRSIDAAVDITNYVMLELGQPLHAFDLAEINGGIRVRMAEEGEKLVLLDGQEVTLRSDT
LVIADHSRALAIAGVMGGEHSGVSTTTRDVFLESAFFDQIAVAGKARSYGLHTDASHRYE
RGVDWQLAREAMERATGLLLDITGGEAGPIIETVSEQHLPKIAPITLRAQRITQMLGMEM
DAAEVERLLSALGLGISADGAGQWRVEVPSHRFDISLEVDLIEELARLYGYNRLPVRYPQ
ARLAPQAKAEARSDLPELRRLLVARGYQEAITYSFIDPRQFELFNPGVEPLLLANPISND
MAAMRSSLWPGLVKSLQHNLNRQQDRVRLFESGLRFVGQLDGLKQESMLAGVVCGSRLPE
GWAQGRDGVDFFDVKADVEAVLGFAGALDAFTFVPGKHPALHPGQTARIERDGREVGYVG
AIHPELSKTLGLDRPVFVFELVLAEVAQGKMPKFQELSRFPEVRRDLALLADRDVASSAV
LEVIRENAGEWLTDLRLFDVYQGKGIDPHRKSLAVGLTWQHPSRTLNDDEVNTATQNILT
SLENRLNATLRK