Protein Info for Pf6N2E2_1751 in Pseudomonas fluorescens FW300-N2E2

Annotation: Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 16 to 472 (457 residues), 540.9 bits, E=1.1e-166

Best Hits

Swiss-Prot: 58% identical to ALDA_ECOLI: Lactaldehyde dehydrogenase (aldA) from Escherichia coli (strain K12)

KEGG orthology group: K07248, lactaldehyde dehydrogenase / glycolaldehyde dehydrogenase [EC: 1.2.1.21 1.2.1.22] (inferred from 83% identity to pmk:MDS_2679)

MetaCyc: 77% identical to NAD+-dependent L-lactaldehyde dehydrogenase (Azotobacter vinelandii NBRC 102612)
Lactaldehyde dehydrogenase. [EC: 1.2.1.22]

Predicted SEED Role

"Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21)" in subsystem L-rhamnose utilization or Methylglyoxal Metabolism (EC 1.2.1.21, EC 1.2.1.22)

MetaCyc Pathways

• superpathway of glycol metabolism and degradation (7/7 steps found)
• ethylene glycol degradation (2/2 steps found)
• L-lactaldehyde degradation (aerobic) (1/2 steps found)
• lactate biosynthesis (archaea) (3/5 steps found)
• D-arabinose degradation II (2/4 steps found)
• methylglyoxal degradation IV (1/3 steps found)
• methylglyoxal degradation V (1/3 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• L-rhamnose degradation II (3/8 steps found)
• superpathway of fucose and rhamnose degradation (5/12 steps found)
• superpathway of pentose and pentitol degradation (15/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.21, 1.2.1.22

Use Curated BLAST to search for 1.2.1.21 or 1.2.1.22

Sequence Analysis Tools

See A0A165Z3M7 at UniProt or InterPro

Protein Sequence (477 amino acids)

>Pf6N2E2_1751 Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21) (Pseudomonas fluorescens FW300-N2E2)
MSHIIYQNYIANAFVASDEHLEVHNPANGQLLGRVPQGSTAEVEQAIAAARQAQRAWAAR
PAIERAGYLRKIASKVREHGERLARTITAEQGKVLELARVEVNFTADYLDYMAEWARRLE
GEVLSSDRAGESIFLLRKPLGVVAGILPWNFPFFLIARKMAPALLTGNTIVIKPSEETPI
NCFEFARLVAETDLPAGVFNVVCGTGATVGHALSGHPGIDLISFTGSVGTGSRIMAAAAP
NITKLNLELGGKAPAIVLADADLDLAIRAITASRVINTGQVCNCAERVYVERKVADAFID
GIAASMAATRYGDPLAEHGLDMGPLINRAALDKVAQMVRTASGQGAQIITGGAVADLGQG
FHYQPTVLAGCSAKMEIMRKEIFGPVLPIQIVDDLDEAIALANDSEYGLTSSIYTASLSA
AMQATRLLDFGETYINRENFEAMQGFHAGTRKSGIGGADGKHGLYEYTHTHVVYIQA