Protein Info for Pf6N2E2_1742 in Pseudomonas fluorescens FW300-N2E2

Annotation: Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 48% identical to GATP1_ORYSJ: Gamma-aminobutyrate transaminase 1, mitochondrial (OSL2) from Oryza sativa subsp. japonica

KEGG orthology group: None (inferred from 54% identity to agr:AGROH133_14247)

MetaCyc: 47% identical to furostanol 26-aldehyde transaminase (Solanum lycopersicum)
2.6.1.-

Predicted SEED Role

"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)

MetaCyc Pathways

biotin biosynthesis I (13/15 steps found)
biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
solasodine and soladulcidine biosynthesis (1/10 steps found)
α-tomatine biosynthesis (1/14 steps found)
superpathway of glycoalkaloid metabolism (Solanaceae) (1/20 steps found)

KEGG Metabolic Maps

Biotin metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.62

Use Curated BLAST to search for 2.6.1.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GUH0 at UniProt or InterPro

Protein Sequence (463 amino acids)

>Pf6N2E2_1742 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) (Pseudomonas fluorescens FW300-N2E2)
MMQETTPPLTELARTDMRFHLHSQTNLRAHQQHGPLVIERGSGIYVTDEHGRDYIEGMSG
LWCAGLGFSNPRLVQAAQRQMAVLPYYHTFNHRVPNVVAQLAERIASLVPFEKPKVFFAC
SGSEANDSMVKLAWAYHRARGNDGKRKIIAHQKGFHGSTVMGASLSGLPNMHAAFGLPLE
NSVLHVQCPHFYRHGIEGESEAQFTERLLRDLEQRIEAEGADTIAAFISEPVMGAGGVIV
PPPGYFVGVQALLKKHDILFLADEIICGFGRTGRWFGHQTLGFTPDMMACAKSLSSGYQP
ISCVVVAENVYQVIEEQSQQLGGFGHGFTYSGHPVAAAVALETLTLYQEMHLPQRTEELG
RLLHQQLEPLLAHPLIGEVRGIGLVAGLELVVDKHTRAPFPREIAIGVQVERAAREQGLI
VRNMGDSIALAPPFIISAEEIVELVARLTRALDAVALANGLSL