Protein Info for Pf6N2E2_1741 in Pseudomonas fluorescens FW300-N2E2

Annotation: Transketolase, N-terminal section (EC 2.2.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00456: Transketolase_N
  • amino acids 30 to 271 (242 residues), 123.3 bits, E=1.7e-39
• PF13292: DXP_synthase_N
  • amino acids 129 to 199 (71 residues), 24.4 bits, E=2.5e-09
• PF00676: E1_dh
  • amino acids 132 to 237 (106 residues), 27.5 bits, E=2.4e-10

Best Hits

KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 69% identity to bac:BamMC406_4664)

MetaCyc: 36% identical to 6-deoxy-6-sulfo-D-fructose transketolase subunit A (Clostridium sp. MSTE9)
RXN-22969 [EC: 2.2.1.15]; 2.2.1.15 [EC: 2.2.1.15]

Predicted SEED Role

"Transketolase, N-terminal section (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• Bifidobacterium shunt (14/15 steps found)
• pentose phosphate pathway (8/8 steps found)
• Rubisco shunt (9/10 steps found)
• Calvin-Benson-Bassham cycle (11/13 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• oxygenic photosynthesis (12/17 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (6/9 steps found)
• sulfoquinovose degradation V (3/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Carbon fixation in photosynthetic organisms
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.1

Use Curated BLAST to search for 2.2.1.1 or 2.2.1.15

Sequence Analysis Tools

See A0A159ZVT9 at UniProt or InterPro

Protein Sequence (294 amino acids)

>Pf6N2E2_1741 Transketolase, N-terminal section (EC 2.2.1.1) (Pseudomonas fluorescens FW300-N2E2)
MPAANLAVRDPVTPLTPEQIARLKERALFVRLETVRLISIAKVGHYTSVFSCAEIFSALY
YDTLRLKRGEPKWTGRDRFLMGKGHAAVGLYPLLADWDFFDKAWLDDYTRLGSPLGDHPD
MRKVPGVDFSSGSIGHALSGGLGMALGGRLRGNVFDVYVLLGDGEMQEGQVWEAALAAAH
HKTGNLIAIVDRNGYQLDGKVDDVVGIEPLADKWRAFGWDVREVDGHDVAAVAMLFRELK
AEPARERPVCVIAITAKGKGVEYMETEPGWHLGWLAPEDEVLAIEEIKANGGVQ