Protein Info for Pf6N2E2_1739 in Pseudomonas fluorescens FW300-N2E2
Annotation: Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01845, glutamate-1-semialdehyde 2,1-aminomutase [EC: 5.4.3.8] (inferred from 66% identity to agr:AGROH133_14224)Predicted SEED Role
"Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 5.4.3.8)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- superpathway of heme b biosynthesis from glutamate (10/10 steps found)
- tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.4.3.8
Use Curated BLAST to search for 5.4.3.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZY07 at UniProt or InterPro
Protein Sequence (427 amino acids)
>Pf6N2E2_1739 Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8) (Pseudomonas fluorescens FW300-N2E2) MSANQAWYARAEKVLPGGHLSPSRKLGQPYAFVRAEGAYLFDADGHRHTDFHCGFGAHVL GHGHPAIRDRVFEVAQRMDLMGAGITDLEVEVAETLVRYIPCAEQIAFCNSGSEATYHAL RLARAATGRKRIIKFQGGYHGWHDYVAMNGQTARDKIGQFDPMSDGILHAAAEYTAVLPY NDAEAVERYLKAHPGEVAAIIVEPIAHNMGAVAATDAFLKDLRSLTRTHGVVLIFDEVIT GFRHALGGYQSIVGVTPDLATFGKAASSGYPVGFIAGKRELMHLIGMAGPKGVFMGGTCN GTPSTLAAVQATLEELARPGTYERLFELGDYMRTQLDAIVQRLGIAAQSAGYGSAWLLYF FEGPYCQYTDLLRNDDALDMAFRQRLIEKRHIFQPMQLKRLYLSTAHTREILDESLGVIE DVLKALV