Protein Info for Pf6N2E2_1728 in Pseudomonas fluorescens FW300-N2E2

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1003 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 276 to 296 (21 residues), see Phobius details PF13185: GAF_2" amino acids 390 to 534 (145 residues), 58.2 bits, see alignment E=2.2e-19 PF01590: GAF" amino acids 392 to 533 (142 residues), 54.1 bits, see alignment E=5.1e-18 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 545 to 706 (162 residues), 146.7 bits, see alignment E=2.7e-47 PF00990: GGDEF" amino acids 547 to 703 (157 residues), 167.8 bits, see alignment E=3.5e-53 PF00563: EAL" amino acids 726 to 958 (233 residues), 252.3 bits, see alignment E=8.2e-79

Best Hits

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a2263)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXZ9 at UniProt or InterPro

Protein Sequence (1003 amino acids)

>Pf6N2E2_1728 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens FW300-N2E2)
MLDVTSRLGRQLLPQSLRAQFTLAFLTLALLILAGGATAVYALRTSNSATRQLTDERLVR
MQNGQDMVQRTLLIERQTDQLLTTRSSDILRSSYAATVEQLEALDQLVQQLTAANSGVAI
LDMHQSSQMFRNTANIVAQLRESLLQTEVTFEQALADHTARLTAAQTRASLELAVLLFDL
PQAVDSEAVQRLRARYERLPRTVGALSDAEVQIPDLFSLRQTLINQHNVLQRFNEQLKNE
AGVLVAVARTQSNAYTQDYREAVQRLVEASNRSQRWVLIMLGASLVFAWFVTRVFMGRHV
LVRLNEISRQLRQEHTDKTHLMMADHGKDEIGNMARAVNQFLEDRLQLEKRTAQLCIATE
RLAVQNDRLEQEAIVRAGQGHVLELIARSTELAEVLDSLAHLVESQLEGMKVSILLLDEE
GKHLLHGTAPSLPKAYSQLIDGIEIGPNVGSCGTSAYRREPVIVTDIEQDPLWEAYRSLV
APYGFRACWSTPILSHERKVLGTFALYANTVRSPSAAETQLIDMATPLAGIAIERQLTEK
RIRFMGDHDALTGLPNRTLLEDRLKQAMLYAQRYNRLVTVVFLDLDKFKLVNDSLGHSAG
DELLKTVAQRMLGCVRRTDTVVRLGGDEFVIILFDQPSDLDGVTPVLHKIQEAILRPIQL
SGHTLHVTCSMGLATYPTDGTDTDTLLSNADAAMYRAKELGRNSFQFYTSEMNNKVQGKL
AMQDGLRSALNNDEFLLLYQPQVDLQSGQIIGVEALIRWQHPELGMVSPIKFIPQAEETG
LIVPIGDWVIHSACRQNKAWQDAGWPPITMSVNISARQFIERDLIDRVMHALQESGLDPM
YLELELTESLIMQDLQQAISKMKALQAMGISLSIDDFGTGYSSLAALKSFPIARLKIDQS
FVRDLPDNENDKAIATAVISLGHKLNLKVIAEGVETEEQQTFLRDNGCDEIQGYFFSKAV
SAQEIGLLLRMPRLPHASRIASPGSPRQNKRDVKRPSGPVSGH