Protein Info for Pf6N2E2_1726 in Pseudomonas fluorescens FW300-N2E2

Annotation: 3-methylmercaptopropionyl-CoA ligase (DmdB)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 25 to 402 (378 residues), 260.9 bits, E=1.8e-81
• PF13193: AMP-binding_C
  • amino acids 452 to 527 (76 residues), 78.7 bits, E=5.1e-26

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a2265)

MetaCyc: 56% identical to short chain acyl-CoA synthetase (Pseudomonas putida KT2440)
Butyrate--CoA ligase. [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]; 6.2.1.2 [EC: 6.2.1.2]

Predicted SEED Role

"3-methylmercaptopropionyl-CoA ligase (DmdB)"

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• fatty acid salvage (6/6 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• valproate β-oxidation (7/9 steps found)
• octane oxidation (4/5 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• 4-oxopentanoate degradation (4/9 steps found)
• cannabinoid biosynthesis (6/13 steps found)
• sporopollenin precursors biosynthesis (4/18 steps found)

KEGG Metabolic Maps

• Butanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.2

Sequence Analysis Tools

See A0A159ZVN2 at UniProt or InterPro

Protein Sequence (540 amino acids)

>Pf6N2E2_1726 3-methylmercaptopropionyl-CoA ligase (DmdB) (Pseudomonas fluorescens FW300-N2E2)
MSIYEQGFAPTGVNHTALTPLSFIERTASVYPDYPAVIHGSIRRTWADTYRRCRRLASAL
AGRGIGKNDTVAVMLPNIPAMLEAHFGVPMIGAVLNALNVRLDAEAIAFMLAHGEAKVLI
ADREFHDVVQAAIGMLDHPPLVIDLDDPEYGEGQAVSELDYEAFLAEGDPDFAWQWPDDE
WQAISLNYTSGTTGNPKGVVYHHRGAYLNSLGNQMTWAMGNHPVYLWTLPMFHCNGWCYP
WTVTALAGVHVFLRRVDPQKILNLIREHQITHLCGAPIVLNALVNMPDSAKAAIDHPVSA
MVAGAAPPAKVIGAVEEMGIKVTHVYGLTEVYGPVTLCAWHAAWDELPLEQRAQIKARQG
VRYPTLEGLMVADPRTLEPTPHDGQTIGEIFMRGNTVMKGYLKNPSATAEAFEGGWFHTG
DLAVTHADGYVEIRDRLKDIIISGGENISTIELEGVLYRHPAVLEAAVVARPDEKWGETP
CAFITLKSDHTDVREAEIISFCREHLAGFKVPRTVVFTQLPKTSTGKIQKFVLRDMAKNL