Protein Info for Pf6N2E2_1697 in Pseudomonas fluorescens FW300-N2E2

Annotation: Alkanesulfonate monooxygenase (EC 1.14.14.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 PF00296: Bac_luciferase" amino acids 4 to 325 (322 residues), 295.9 bits, see alignment E=2e-92 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 4 to 349 (346 residues), 603.9 bits, see alignment E=4e-186

Best Hits

Swiss-Prot: 67% identical to SSUD_PSE14: Alkanesulfonate monooxygenase (ssuD) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 97% identity to pba:PSEBR_a2292)

MetaCyc: 68% identical to alkanesulfonate monooxygenase subunit (Pseudomonas putida DS1)
RXN-18086 [EC: 1.14.14.34]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.5

Use Curated BLAST to search for 1.14.14.34 or 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z3A7 at UniProt or InterPro

Protein Sequence (376 amino acids)

>Pf6N2E2_1697 Alkanesulfonate monooxygenase (EC 1.14.14.5) (Pseudomonas fluorescens FW300-N2E2)
MSLDIFWFLPTSGDTRYLGHSGSGRPATNAYMRQIAVAADQLGYDGLLIPTGASCLDPWV
TAASLVPVTQRIKLLVALRTSLGNPTASARQAASLDQASGGRLLLNVVPGGDATELEADG
VFLTHDERYDASDEFLSIWRRLLQGETVDFDGKHLQVRGAQNLFPPVQQPHPPLYFGGSS
EAAHELAAKHVDAYLSWGEPPAAVAEKIADVRARAARHGRSVRFGVRLHVIVRETSEAAW
AAADELISHLDDATIAAAQANYGKMDSEGQRRMAALHGGDRNKLEVAPNLWAGVGLVRGG
AGTALVGDPDTVAARLLEYADLGVDSFVLSGYPHLEEAYRFAELVFPLLPGKGKVTVEGA
FTGGAFDVRAGKEKVA