Protein Info for Pf6N2E2_165 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribokinase (EC 2.7.1.15)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 PF00294: PfkB" amino acids 3 to 295 (293 residues), 248.5 bits, see alignment E=1e-77 TIGR02152: ribokinase" amino acids 5 to 300 (296 residues), 373.7 bits, see alignment E=3.3e-116 PF08543: Phos_pyr_kin" amino acids 180 to 278 (99 residues), 39.5 bits, see alignment E=4.5e-14

Best Hits

Swiss-Prot: 44% identical to RBSK_ECO57: Ribokinase (rbsK) from Escherichia coli O157:H7

KEGG orthology group: K00852, ribokinase [EC: 2.7.1.15] (inferred from 97% identity to pba:PSEBR_a3707)

MetaCyc: 44% identical to ribokinase (Escherichia coli K-12 substr. MG1655)
Ribokinase. [EC: 2.7.1.15]

Predicted SEED Role

"Ribokinase (EC 2.7.1.15)" in subsystem D-ribose utilization or Deoxyribose and Deoxynucleoside Catabolism (EC 2.7.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YLE0 at UniProt or InterPro

Protein Sequence (304 amino acids)

>Pf6N2E2_165 Ribokinase (EC 2.7.1.15) (Pseudomonas fluorescens FW300-N2E2)
MPAKVVVIGSLNMDLVTRAPRLPRGGETLIGESFSTIPGGKGANQAVAAARLGAEVSMVG
CVGNDAYGEQLRGGLLAEGIDCQAVSVVEGASGVALIVVDDNSQNAIVIVAGANGALTAE
VLDSVDEVLQSADVIICQLEVPDATVGHALKRARELGKIVILNPAPASHTLPADWYACID
YLVPNESEAAVLSGLAVDSLETAEAAAAHLIAAGAGKVIVTLGAQGLMFANGVSFEHFPA
PRVKAVDTTAAGDTFVGGFAAALASGKSEVDAIRFGQAAAALSVTRAGAQPSIPTLLEVQ
AFKS