Protein Info for Pf6N2E2_1623 in Pseudomonas fluorescens FW300-N2E2

Annotation: Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR00709: 2,4-diaminobutyrate 4-transaminase" amino acids 32 to 469 (438 residues), 562.5 bits, see alignment E=2.7e-173 PF00202: Aminotran_3" amino acids 49 to 461 (413 residues), 303 bits, see alignment E=1.5e-94

Best Hits

KEGG orthology group: K00836, diaminobutyrate-2-oxoglutarate transaminase [EC: 2.6.1.76] (inferred from 68% identity to reh:H16_B1692)

MetaCyc: 66% identical to L-2,4-diaminobutyrate:2-ketoglutarate 4-aminotransferase monomer (Pseudomonas aeruginosa PAO1)
Diaminobutyrate--2-oxoglutarate transaminase. [EC: 2.6.1.76]

Predicted SEED Role

"Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76)" (EC 2.6.1.76)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.76

Use Curated BLAST to search for 2.6.1.76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZW09 at UniProt or InterPro

Protein Sequence (476 amino acids)

>Pf6N2E2_1623 Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76) (Pseudomonas fluorescens FW300-N2E2)
MESVQPRLSSSATFTLEQFRQQAPRELNTNPYLQRQAARESNARSYPRRIPLALQEAHGL
YVRDTQGQLFMDCLAGAGTLALGHNHPVAIAAMRQTLDSGLPLHTLDLTTPVKDRFVEDL
FNALPENFARHARIQFCGPTGADGIEAALKLARTATGRKPILSFSGGYHGMTLGTLSLMG
NLGPKQALGSLMADVQFLPYPYDYRCPFGIGGEAGVDAGLHFIEQLLSDPESGVLPPAAV
VVEVVQGEGGVIPAPIRWLQGLRQLTRKFGVALIIDEVQTGLGRTGKLFAFEHADIEPDI
LVLSKAIGGGLPLAVMVYREELDTWKPGAHAGTFRGNQMAMAAGAATLRHIISEDLPGHA
DVMGQRLMAALRQLQDRYACLGQVRGRGLMVGVEIVSDTASDSRVPAADTALAQAIQRQC
LRLGVILELGGRHGAVVRFLPPLIIQAEEVDVLVELFQVALANALVEVKGRTLHIA