Protein Info for Pf6N2E2_1599 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cytochrome c-type protein NapC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 198 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details TIGR02161: periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family" amino acids 8 to 193 (186 residues), 337.6 bits, see alignment E=1e-105 PF03264: Cytochrom_NNT" amino acids 18 to 192 (175 residues), 262.2 bits, see alignment E=5.1e-82

Best Hits

Swiss-Prot: 71% identical to NAPC_RHOS4: Cytochrome c-type protein NapC (napC) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K02569, cytochrome c-type protein NapC (inferred from 98% identity to pba:PSEBR_a2088)

MetaCyc: 58% identical to NapC (Aliivibrio fischeri)
RXN-15816 [EC: 7.1.1.8]

Predicted SEED Role

"Cytochrome c-type protein NapC" in subsystem Nitrate and nitrite ammonification or trimethylamine N-oxide (TMAO) reductase

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.1.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZTT4 at UniProt or InterPro

Protein Sequence (198 amino acids)

>Pf6N2E2_1599 Cytochrome c-type protein NapC (Pseudomonas fluorescens FW300-N2E2)
MKALFALLKDYWGVLRRPSMYYSLGFLTLGGFIAGIVFWGGFNTALEATNTEAFCVSCHE
MRDNVFVELKDTIHYTNRSGVRASCPDCHVPHEWTHKIARKMQASKEVWGKIFGTIDTRD
KFLALRRELAEHEWARLKANDSRECRNCHNFEFMDFTRQGKRAASMHSTSLANGEATCID
CHKGIAHKLPDMSGVKGW