Protein Info for Pf6N2E2_149 in Pseudomonas fluorescens FW300-N2E2

Annotation: Inner membrane component of tripartite multidrug resistance system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 509 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 52 to 72 (21 residues), see Phobius details amino acids 81 to 101 (21 residues), see Phobius details amino acids 108 to 131 (24 residues), see Phobius details amino acids 139 to 161 (23 residues), see Phobius details amino acids 167 to 186 (20 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 232 to 251 (20 residues), see Phobius details amino acids 272 to 291 (20 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details amino acids 334 to 354 (21 residues), see Phobius details amino acids 360 to 385 (26 residues), see Phobius details amino acids 398 to 421 (24 residues), see Phobius details amino acids 474 to 494 (21 residues), see Phobius details PF06609: TRI12" amino acids 7 to 267 (261 residues), 27.5 bits, see alignment E=1.7e-10 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 14 to 493 (480 residues), 503.2 bits, see alignment E=3.9e-155 PF07690: MFS_1" amino acids 19 to 413 (395 residues), 176.9 bits, see alignment E=8.3e-56 PF00083: Sugar_tr" amino acids 24 to 184 (161 residues), 34.7 bits, see alignment E=1.5e-12

Best Hits

Swiss-Prot: 53% identical to EMRB_ECOLI: Multidrug export protein EmrB (emrB) from Escherichia coli (strain K12)

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 94% identity to pfl:PFL_3916)

MetaCyc: 53% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YKU2 at UniProt or InterPro

Protein Sequence (509 amino acids)

>Pf6N2E2_149 Inner membrane component of tripartite multidrug resistance system (Pseudomonas fluorescens FW300-N2E2)
MSNNASFTPPSLLLSTIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAV
SNAIALPLTGWLSRRFGEVKLFLWATMLFVLASFLCGISTSMPELIGFRVLQGLVAGPLY
PMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINVPIGVF
AVLVVQQQLKARPVVTSHQPMDYVGLITLIIGVGALQVILDKGNDLDWFESNFIIIGALI
SIIALAVFVIWEMTDRHPVVNLRLFAYRNFRIGTIVLVGGYAGFFGINLILPQWLQTQMG
YTATWAGLAVAPIGILPVLMSPFVGKYAHRFDLRVLAGLAFLAIGLSCFMRAGFTNEVDF
QHIALVQLFMGIGVALFFMPTLSILMSDLPPHQIADGAGLATFLRTLGGSFAASLTTWIW
IRRADLHHAYLSESISTFEPATREALNQLGGASTPAYARLDQVLTSQAYMLSTVDYFTLL
GWAFMGLIVLVWLAKPPFAAKAGPASAGH