Protein Info for Pf6N2E2_1468 in Pseudomonas fluorescens FW300-N2E2

Annotation: NAD-dependent formate dehydrogenase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 transmembrane" amino acids 199 to 221 (23 residues), see Phobius details PF01512: Complex1_51K" amino acids 145 to 315 (171 residues), 152.6 bits, see alignment E=8.3e-49 PF10589: NADH_4Fe-4S" amino acids 427 to 510 (84 residues), 84.8 bits, see alignment E=3.2e-28

Best Hits

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 98% identity to pba:PSEBR_a2582)

Predicted SEED Role

"NAD-dependent formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z1L7 at UniProt or InterPro

Protein Sequence (519 amino acids)

>Pf6N2E2_1468 NAD-dependent formate dehydrogenase beta subunit (Pseudomonas fluorescens FW300-N2E2)
MLTLCIPRDSVARAVGADKVADALALEAERRQLPLTVLRTSSRGLYWLEPLVELESAGVR
LGFGPVTPADVPGLLDALTGDPGSHPLALGPVEAIPYLKSQQRLLFARAGITQPLSLDDY
RAQGGFQGLARSIQMDGADVVASVLDSGLRGRGGAAFPAGIKWRTVRDAVGAQKYVVCNA
DEGDSGTFADRMLMEGDPFLLIEGMIIAGLAVGATMGYIYVRSEYPAAISTLNDALRIAR
DAGYLGANVSGSGRTFDIEVRVGAGAYICGEETALLESLEGKRGTVRAKPPLPAIQGLFG
LPTLVHNVVTLASVPIILEKGAQFYRDFGMGRSLGTMPFQLAGNVRQGGLVERAFGLSLR
ELVEGYGGGTASGRPLKAAQVGGPLGAWVPPSQFDTPLDYEAFAAMGAMLGHGGVVVADD
SLDMAQMARFALQFCAEESCGKCTPCRIGSTRGVEVVDRLIASTEAGARQEQALLLQDLC
DTLQYGSLCALGGMTSYPVASALKHFPADFGLATTEADQ