Protein Info for Pf6N2E2_1467 in Pseudomonas fluorescens FW300-N2E2

Annotation: NAD-dependent formate dehydrogenase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 PF13510: Fer2_4" amino acids 23 to 98 (76 residues), 81.8 bits, see alignment E=1.2e-26 PF10588: NADH-G_4Fe-4S_3" amino acids 105 to 144 (40 residues), 60.5 bits, see alignment (E = 3.5e-20) PF13237: Fer4_10" amino acids 166 to 225 (60 residues), 25.7 bits, see alignment 3.7e-09 PF12838: Fer4_7" amino acids 170 to 227 (58 residues), 33.5 bits, see alignment 1.9e-11 PF00037: Fer4" amino acids 212 to 227 (16 residues), 22.9 bits, see alignment (E = 2.3e-08) PF04879: Molybdop_Fe4S4" amino acids 243 to 293 (51 residues), 58.4 bits, see alignment 2.3e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 246 to 921 (676 residues), 931.3 bits, see alignment E=1.3e-284 PF00384: Molybdopterin" amino acids 298 to 729 (432 residues), 234.6 bits, see alignment E=7e-73 PF01568: Molydop_binding" amino acids 813 to 918 (106 residues), 99.4 bits, see alignment E=5e-32

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 78% identity to bam:Bamb_0895)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXG6 at UniProt or InterPro

Protein Sequence (958 amino acids)

>Pf6N2E2_1467 NAD-dependent formate dehydrogenase alpha subunit (Pseudomonas fluorescens FW300-N2E2)
MINIFDPSTDIDLGTPARESDVQVSLTIDGREISVASGTSVMRAAALLGTTIPKLCATDS
LEAFGSCRMCLVEIDGMRGYPASCTTPVTEGMVVRTQTSKLATLRRNVMELYISDHPLDC
LTCPANGNCELQTVAGQVGLREVRYGYEGANHLAEAKDVSNPYFDYDPSKCIVCNRCVRA
CEEIQGTFALTITGRGFDSRVEAAGGDNFLDSECVSCGACVQACPTATLIDKSVVEIGQP
ERSVITTCAYCGVGCSFRAEMKGEQLVRMVPDKNGQANHGHSCVKGRFAWGYATHPDRIT
KPMIRKHINDPWQEVSWDEAVTYAASELRRIQLKYGRDSIGGITSSRCTNEETYLVQKLV
RTAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQNFDSVMKADVILVMGANPTDAHPVF
GSQLKRRLRQGARLIVIDPRRIDLVDSPHARAELHLQLRPGTNVAMLNALGHVIATEGLI
DQPFVDARCEAADFARWRDFVSQPENSPDVLGPVCGVPAEQIRAAARLYATGGNAAIYYG
LGVTEHSQGSTSVMGIANLAMATGNIGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRH
VSNETVRAQFEQAWNVTLQPDPGLRIPNMFEAALDGTFKALYCQGEDIAQSDPNTQHVTA
ALSALECVIVQDIFLNETAKFAHVFLPGSSFLEKDGTFTNAERRISRVRKVMDPLAGKAD
WEATIALADALGYKMHYNHPSQIMDEIARLTPTFSRVSYAELDRHGSLQWPCNDAAPDGT
PTMHIDQFVRGKGRFMLTGYVPTEEKVNSRYPLLLTTGRILSQYNVGAQTRRTDNVAWHE
EDRLEIHPTDAENRGIVDEDWVGIGSRAGQTVLRAKVTERVAPGVVYTTFHFPESGANVI
TTDNSDWATNCPEYKVTAVEIVRVSQPSEWQKRYQAFSDEQSRLLRERRHAEKAEVRR