Protein Info for Pf6N2E2_1463 in Pseudomonas fluorescens FW300-N2E2

Annotation: Isochorismate pyruvate-lyase (EC 4.-.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 75 92 TIGR01803: chorismate mutase related enzymes" amino acids 1 to 81 (81 residues), 77.7 bits, see alignment E=2.9e-26 PF01817: CM_2" amino acids 5 to 81 (77 residues), 61.7 bits, see alignment E=3.5e-21

Best Hits

Swiss-Prot: 45% identical to PCHB_PSEAE: Isochorismate pyruvate lyase (pchB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K04782, isochorismate pyruvate-lyase [EC: 4.1.3.-] (inferred from 96% identity to pba:PSEBR_a2587)

MetaCyc: 45% identical to isochorismate pyruvate lyase (Pseudomonas aeruginosa)
RXN-1981 [EC: 4.2.99.21]

Predicted SEED Role

"Isochorismate pyruvate-lyase (EC 4.-.-.-)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. (EC 4.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-

Use Curated BLAST to search for 4.-.-.- or 4.1.3.- or 4.2.99.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXD5 at UniProt or InterPro

Protein Sequence (92 amino acids)

>Pf6N2E2_1463 Isochorismate pyruvate-lyase (EC 4.-.-.-) (Pseudomonas fluorescens FW300-N2E2)
MEDIRREIDALDQAVITLLGKRYQYVLAASKFKTSVTSVQAPERLKAMLATRREWAEAEG
LSPDAIEKLYSDLVHHFIAEEMKRWTANQTHN