Protein Info for Pf6N2E2_1420 in Pseudomonas fluorescens FW300-N2E2

Annotation: ABC exporter for hemopore HasA, membrane fusion protein (MFP) family component HasE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 transmembrane" amino acids 34 to 56 (23 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 34 to 456 (423 residues), 358.7 bits, see alignment E=2.2e-111 PF00529: CusB_dom_1" amino acids 47 to 408 (362 residues), 27.2 bits, see alignment E=6.1e-10 PF13533: Biotin_lipoyl_2" amino acids 77 to 117 (41 residues), 27.8 bits, see alignment 3.5e-10 PF13437: HlyD_3" amino acids 306 to 407 (102 residues), 48.3 bits, see alignment E=3.1e-16

Best Hits

Swiss-Prot: 38% identical to APRE_PSEAE: Alkaline protease secretion protein AprE (aprE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12537, protease secretion protein HasE (inferred from 99% identity to pba:PSEBR_a2635)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z157 at UniProt or InterPro

Protein Sequence (456 amino acids)

>Pf6N2E2_1420 ABC exporter for hemopore HasA, membrane fusion protein (MFP) family component HasE (Pseudomonas fluorescens FW300-N2E2)
MRDLTPGAQAYSEQALSVRSTTTLPSASTDAGSAARYGVGFLVLTLGGFLLWACLAPLDQ
GVVGSGTVVVAGERKAVQSLVGGVVEKLLVSDGDRVTQGQLLVQLNTVQAQSQLDVTLGK
LLNDRSIEARLIAERLGNAEIQWPAELLAHADEPRVKAAMALQSQLFITRRAELGSRLQI
IEHEAAALQQQLLGYEGVKRNYDAQMRFQQQELEGLRDLAREGYVPRNKLFEAERNAAQL
AGQIASGVGDVGRTRQAINESRLKALQAQQEFRRDAETQLSEVSAEAAGYADQIRALQFE
VDNGAIRAPVAGQVMDVSIHTVGGVAQAGQTLMQVVPLDAPMAITARFEPLMANKLRPGL
PVHVHFTALQRVDTPTVTGTVTTVSADQLINEQTHQPYFSAKVEIPADTVVELQSAGLLI
RPGMLADVTVVTGERTLMNYLMKPLRERLLAAFKEE