Protein Info for Pf6N2E2_1389 in Pseudomonas fluorescens FW300-N2E2

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 29 to 581 (553 residues), 781.5 bits, see alignment E=2e-239 PF13570: PQQ_3" amino acids 76 to 116 (41 residues), 19.1 bits, see alignment 2.1e-07 amino acids 117 to 165 (49 residues), 22.4 bits, see alignment 2e-08 amino acids 505 to 545 (41 residues), 27.5 bits, see alignment 5e-10 PF13360: PQQ_2" amino acids 90 to 237 (148 residues), 56.3 bits, see alignment E=5.7e-19 amino acids 495 to 563 (69 residues), 37.6 bits, see alignment E=3e-13 PF01011: PQQ" amino acids 98 to 124 (27 residues), 25.5 bits, see alignment (E = 1.2e-09) amino acids 147 to 181 (35 residues), 24.8 bits, see alignment (E = 2.1e-09) amino acids 527 to 563 (37 residues), 41.1 bits, see alignment 1.4e-14

Best Hits

Swiss-Prot: 89% identical to QEDH_PSEPU: Quinoprotein alcohol dehydrogenase (cytochrome c) (qedA) from Pseudomonas putida

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a2660)

MetaCyc: 92% identical to quinoprotein alcohol dehydrogenase (Pseudomonas putida U)
1.1.2.-

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZTD7 at UniProt or InterPro

Protein Sequence (618 amino acids)

>Pf6N2E2_1389 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8) (Pseudomonas fluorescens FW300-N2E2)
MRIKTLPALSSLTVALLLAGSLSTSPLARAAVTWEDIANDHLTTQDVLQYGMGTNAQRWS
PLAQVNDKNVFKLTPAWSYSFGDEKQRGQESQAIVSDGVVYVTGSYSRVFALDAKTGKRL
WTYNHRLPDNIRPCCDVVNRGAAIYGDKIYFGTLDARVVALDKNTGKVVWNKKFGDHAGG
YTMTGAPVLIKDKTSGKVLLIHGSSGDEFGVVGQLFARDPDTGEEVWMRPFVEGHMGRLN
GKDSTPTGDVKAPSWPDDKTTETGKVEAWSHGGGAPWQSASFDAETNTIIVGAGNPGPWN
TWARTAKDGNPHDYDSLYTSGQVGVDPSTGEVKWFYQHTPNDAWDFSGNNELVLFDYKDK
DGKVVKATAHADRNGFFYVVDRNNGKLQNAFPFVDNITWASHIDLKTGRPVENAGQRPAK
PLPGETKGKPVEVSPPFLGGKNWNPMAYSQDTGLFYVPGNQWKEEYWTEEVNYKKGSAYL
GMGFRIKRMYDDHVGSLRAMNPTTGKVVWEHKEPLPLWAGVLATKGNLVFTGTGDGFFKA
FDAKTGKELWKFQTGSGIVSPPITWEQDGEQFIGVTVGYGGAVPLWGGDMAELTKPVAQG
GSFWVFKIPSWDKATAQK