Protein Info for Pf6N2E2_1382 in Pseudomonas fluorescens FW300-N2E2

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 27 to 556 (530 residues), 803.6 bits, see alignment E=4.1e-246 PF13360: PQQ_2" amino acids 73 to 237 (165 residues), 64.7 bits, see alignment E=1.6e-21 amino acids 470 to 538 (69 residues), 40.4 bits, see alignment E=4.2e-14 PF01011: PQQ" amino acids 95 to 127 (33 residues), 27.9 bits, see alignment (E = 2.2e-10) amino acids 145 to 180 (36 residues), 27.1 bits, see alignment 3.8e-10 amino acids 502 to 538 (37 residues), 37.9 bits, see alignment 1.4e-13 PF13570: PQQ_3" amino acids 114 to 162 (49 residues), 22.2 bits, see alignment 2.4e-08 amino acids 480 to 520 (41 residues), 25.1 bits, see alignment 2.8e-09

Best Hits

Swiss-Prot: 54% identical to EXAA_PSEAE: Quinoprotein alcohol dehydrogenase (cytochrome c) (exaA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 98% identity to pba:PSEBR_a2665)

MetaCyc: 54% identical to alcohol dehydrogenase (cytochrome c550) monomer (Pseudomonas aeruginosa)
RXN-11333 [EC: 1.1.2.8]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZUZ0 at UniProt or InterPro

Protein Sequence (591 amino acids)

>Pf6N2E2_1382 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8) (Pseudomonas fluorescens FW300-N2E2)
MSQPARRQPFAVSLLLSAMLLSGSALAAVTDQEILQDPKNPEQIVTNGLGVQGQRYSPLD
TLNTDNVKDLRPVWAFSFGGEKQRGQQAQPMIKDGVMYLTGSYSRVFAVDARTGKKLWQY
DARLPDDIRPCCDVINRGVALYGDLVFFGTLDAKLVALNKDTGKVVWSKKVADHKEGYSI
SAAPLIVNGKLITGVAGGEFGVVGQISAYDPKNGELLWTRPTVEGHMGYVYKDGKAVENG
ISGGEAGKTWPGDLWKTGGAAPWLGGYYDPETNLLLFGTGNPAPWNSHLRPGDNLYSSSR
LALNPDDGTIKWHFQSTPHDGWDYDGVNELISFNYKEGGKDIKAAATADRNGFFYVLDRT
NGKFIRGFPFVDKITWATGLDKDGRPIYNEASRPGAPGSEAKGSSVFVAPAFLGAKNWMP
MAYNQDTGLFYVPSNEWGMDIWNEGIAYKKGAAFLGAGFTIKPLNEDYIGVLRAIDPKTG
KEVWRHKNYAPLWGGVLTTKGNLVFTGTPEGFLQAFNAKTGEKVWEFQTGSGVLGSPITW
EMDGEQYVSVVSGWGGAVPLWGGEVAKRVKDFNQGGMLWTFKLPKALVAKR