Protein Info for Pf6N2E2_137 in Pseudomonas fluorescens FW300-N2E2
Annotation: Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to GLCD_ECOL6: Glycolate oxidase subunit GlcD (glcD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a3736)MetaCyc: 80% identical to glycolate dehydrogenase, putative FAD-linked subunit (Escherichia coli K-12 substr. MG1655)
Glycolate dehydrogenase. [EC: 1.1.99.14]; 1.1.99.14 [EC: 1.1.99.14]
Predicted SEED Role
"Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)
MetaCyc Pathways
- superpathway of glycol metabolism and degradation (7/7 steps found)
- glycolate and glyoxylate degradation I (4/4 steps found)
- glycolate and glyoxylate degradation II (2/2 steps found)
- glycolate and glyoxylate degradation III (1/3 steps found)
- photorespiration II (6/10 steps found)
- photorespiration I (5/9 steps found)
- photorespiration III (5/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.14
Use Curated BLAST to search for 1.1.99.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165YK95 at UniProt or InterPro
Protein Sequence (511 amino acids)
>Pf6N2E2_137 Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD (Pseudomonas fluorescens FW300-N2E2) MWDSSRPRGIDFMNILYDEHLDGPLPKVDKQALLQALQAQIPDLDILHQEEDLKPYECDG LSAYRTTPLLVALPRRVEQVQALLKLCHGLDVPVVARGAGTGLSGGALPLESGVLLVMAR FNQILHIDPDARTARLQPGVRNLAISQAAAPFGLYYAPDPSSQIACSIGGNVAENAGGVH CLKYGLTVHNLLKLEILTIEGERLTLGSEALDSPGLDLLALFTGSEGLLGVITEVTVKLL PRPQVAKVLLASFDSVDKAGRAVADIIAAGIIPGGLEMMDNLAIRAAEDFIHAGYPVEAE AILLCELDGVEADVHDDCERVRQVLEQAGATEVRQARDEAERLRFWAGRKNAFPAVGRLA PDYYCMDGTIPRRALPEVLQRIASLGAEHGLRVANVFHAGDGNMHPLILFDANQPGELER AETLGGKILELCVQVGGSITGEHGVGREKINQMCTQFNSDELNLFHAVKAAFDPQGLLNP GKNIPTLHRCAEFGAMHIHGGQLPFPELERF