Protein Info for Pf6N2E2_1327 in Pseudomonas fluorescens FW300-N2E2
Annotation: Glutamate synthase [NADPH] large chain (EC 1.4.1.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a2724)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZVD8 at UniProt or InterPro
Protein Sequence (442 amino acids)
>Pf6N2E2_1327 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) (Pseudomonas fluorescens FW300-N2E2) MEHTRRTPLLRNAIGLGIALMVADSSAQAYELYSDEDTTVTGNFLAVYGLFNSRKNYDGT AGGSSWREGFIKYGLSIDQTLGSLGSVYGTANLVSSGTWGDGDAAGLSDGSERTTKFDEA FAGWRSGDLFPALGKDGVDLSYGRQVITLGDGFIINDDGLNLGKGVADGELNRGGAYYLA ARHAFDETAVVRLGGKEGLHGSLMWIKSDNRAQANTEMAAGTLEYTAAPGTLGLTYIHGI DVDDRYASDFQKQREGMDIYSLRGAGNAGIENAHFAFEYAWQDKDAGPEKAWYGEAGYTF ADLPWTPDLTYRYSRYSKDWDSMFNGQNRGYGTWFQGEVAGNYAGPFNSNTAIQHVGLKL KPAETVTIGALFFDYQTLHARDALNLDAQELDLYVEWAVNEHLIITPLVGLYKPEKDADS GGNQVAGNGTNVYSQLVVAVPF