Protein Info for Pf6N2E2_1277 in Pseudomonas fluorescens FW300-N2E2

Annotation: Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 165 PF13302: Acetyltransf_3" amino acids 6 to 140 (135 residues), 53.5 bits, see alignment E=1.1e-17 PF13420: Acetyltransf_4" amino acids 17 to 158 (142 residues), 39 bits, see alignment E=2.1e-13 PF00583: Acetyltransf_1" amino acids 36 to 139 (104 residues), 65.2 bits, see alignment E=1.7e-21 PF13673: Acetyltransf_10" amino acids 39 to 143 (105 residues), 31.8 bits, see alignment E=3.1e-11 PF13508: Acetyltransf_7" amino acids 57 to 140 (84 residues), 49.1 bits, see alignment E=1.5e-16

Best Hits

Swiss-Prot: 38% identical to AAAT_ECOLI: L-amino acid N-acetyltransferase AaaT (aaaT) from Escherichia coli (strain K12)

KEGG orthology group: K03825, putative acetyltransferase [EC: 2.3.1.-] (inferred from 99% identity to pba:PSEBR_a2796)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZVT3 at UniProt or InterPro

Protein Sequence (165 amino acids)

>Pf6N2E2_1277 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) (Pseudomonas fluorescens FW300-N2E2)
MSESSITLVRFTETHIAGVTALYNDPAVTRQVLQMPFQSTEVWRKRLAMDDERLLKLVAL
HEGEVIGNLGLEQFSRVRRAHCGSLGMGVAVAWQGKGVGSMLLAAALDVADNWMNLRRVE
LTVYADNEAAIGLYRKFGFETEGLMRDYAVRDGRWVDTLAMARLR