Protein Info for Pf6N2E2_1270 in Pseudomonas fluorescens FW300-N2E2

Annotation: Heavy metal RND efflux outer membrane protein, CzcC family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR01845: efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family" amino acids 27 to 482 (456 residues), 432.3 bits, see alignment E=1.1e-133 PF02321: OEP" amino acids 83 to 273 (191 residues), 99.6 bits, see alignment E=9.2e-33 amino acids 297 to 481 (185 residues), 146.4 bits, see alignment E=4.2e-47

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a2803)

Predicted SEED Role

"Heavy metal RND efflux outer membrane protein, CzcC family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZUS8 at UniProt or InterPro

Protein Sequence (491 amino acids)

>Pf6N2E2_1270 Heavy metal RND efflux outer membrane protein, CzcC family (Pseudomonas fluorescens FW300-N2E2)
MTDRSPFNLAATLATARGSRVLSLVLCVAMLSACAVGPDYQRPQAAEPVQYKEAQGWRQA
NPSDAMARGAWWELYGDVQLNALVDQLNASNQTVAQADAQYRQARALVRNARGAFFPTVD
LTVGKTRSSQGTGSSSSSLSSSSSGIRDTYSAQAGVSWEADVWGKLRRTLEADEASAQAS
FADLTAMRLSQQSELVQNYLQLRVIDEQKRLLQTTVDNYQRSLKMTENQYRAGVSGKDAV
AQAQNQLKTTQGDLIDLIWQRAQFENAIAVLVGLPPAEFNLAETQDIPSLPQIPVALPSQ
LLERRPDIASAERSVMAANANIGVAKAAYYPDLTLSLSGGYSSSTYDNWVSVPNRFWSVG
PQLAMTLFDGGQRSAEVDRTVAAYDQTVATYRQTVLDGLREVENYMIQLKVLEDEARVRQ
EALDAARESLRLTQNQYRAGLIAYLDVVTVQATALSNERSVLSLQQSRLIASVQLIAALG
GGWDGQLETDE