Protein Info for Pf6N2E2_1250 in Pseudomonas fluorescens FW300-N2E2

Annotation: Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 757 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 228 to 251 (24 residues), see Phobius details amino acids 283 to 305 (23 residues), see Phobius details amino acids 333 to 353 (21 residues), see Phobius details amino acids 367 to 391 (25 residues), see Phobius details amino acids 412 to 432 (21 residues), see Phobius details amino acids 444 to 465 (22 residues), see Phobius details amino acids 480 to 504 (25 residues), see Phobius details amino acids 515 to 538 (24 residues), see Phobius details amino acids 545 to 566 (22 residues), see Phobius details amino acids 587 to 608 (22 residues), see Phobius details amino acids 628 to 649 (22 residues), see Phobius details amino acids 667 to 689 (23 residues), see Phobius details amino acids 715 to 735 (21 residues), see Phobius details PF00115: COX1" amino acids 281 to 723 (443 residues), 185.4 bits, see alignment E=9e-59

Best Hits

KEGG orthology group: K04561, nitric oxide reductase, cytochrome b-containing subunit I [EC: 1.7.2.5] (inferred from 72% identity to hch:HCH_00713)

MetaCyc: 72% identical to nitric oxide reductase (menaquinol) (Cupriavidus necator H16)
RXN-12129 [EC: 1.7.5.2]

Predicted SEED Role

"Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent" in subsystem Denitrification or Flavohaemoglobin or Nitrosative stress (EC 1.7.99.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.2.5, 1.7.99.7

Use Curated BLAST to search for 1.7.2.5 or 1.7.5.2 or 1.7.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZV70 at UniProt or InterPro

Protein Sequence (757 amino acids)

>Pf6N2E2_1250 Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent (Pseudomonas fluorescens FW300-N2E2)
MGEYRKLWWCLIGILGVTFCLLGWFGREIYRQAPPVPERVQSAGGQLLFQSVDILDGQTA
WQSIGGMQLDSIWGHGGYQAPDWTADWLHRELLAWLELAAQQGYGKAYGDIDGDQQALLR
YQLKREYRGNRLVDGTLVLSERRIQAIRQTGDYYDALFGGDPSLSGSRQSFAMKEVTMPD
PARRQQLTHFFFWTAWAAATERDGLNTTYTNNWPHEPLIDNNATVENMVWSVISIVVLLA
GIGFLVWGWAFLRSHEEGAPTASTQDPLSLITLTPSQKALGKYLLLVGALFGFQVMMGGV
TAHYTVEGQDFYGLPLSKWFPYSLTRTWHLQSAMFWIATGFLAAGLFLAPFINGGKDPKY
QKLGVDLLFWALVLVVAGSYTGNFLAIAQLMPAEWSFWLGHQGYEYVDLGRLWQIAKFSG
IVFWLVLMLRAMVPALRQPGDKNLLVLLACSVIAIGLFYGAGLFYGERTNLSVMEYWRWW
VVHLWVEGFFEVFATTALAFIFTSMGLVSKRLATTATLGSASLFMLGGVPGTFHHIYFAG
TSTPVMAVGATFSALEVVPLILLGYEAWENWHLKERAAWMTNIRWPLQFFIATAFWNMLG
AGVFGFLINPPISLYYIQGLNTTPLHAHAALFGVYGSLALGFSLLILRYLRPNLQFSERL
MKTGFWWLNGGLALMIFTSLLPVGILQFYGSASQGLWYARSEAFMQQDLLQTLRWIRTFG
DIVFLVGAFAVIWQITKGLLGTSERAGISTVGIISRP