Protein Info for Pf6N2E2_1229 in Pseudomonas fluorescens FW300-N2E2

Annotation: Serine protease homologue

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1030 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details PF00082: Peptidase_S8" amino acids 78 to 413 (336 residues), 151.5 bits, see alignment E=4.8e-48 TIGR02601: autotransporter-associated beta strand repeat" amino acids 488 to 519 (32 residues), 43.5 bits, see alignment (E = 2.1e-15) PF12951: PATR" amino acids 489 to 518 (30 residues), 42.8 bits, see alignment (E = 6.5e-15) TIGR01414: outer membrane autotransporter barrel domain" amino acids 553 to 1030 (478 residues), 159.6 bits, see alignment E=1.3e-50 PF03797: Autotransporter" amino acids 758 to 1010 (253 residues), 210 bits, see alignment E=8.3e-66

Best Hits

KEGG orthology group: K12685, subtilase-type serine protease [EC: 3.4.21.-] (inferred from 96% identity to pba:PSEBR_a2826)

Predicted SEED Role

"Serine protease homologue"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H4Q1 at UniProt or InterPro

Protein Sequence (1030 amino acids)

>Pf6N2E2_1229 Serine protease homologue (Pseudomonas fluorescens FW300-N2E2)
MKIDNKKQPGQTGRLFLPKALNRALLCALATLGAAQAAPYVESGRAGNPDSWRSSEFNAE
WGLGAINAQQAYAAGYTGKGVKLGIFDQPVYAAHPEFSGTGKVVTLVTSGIREYTDPYIP
VKAGDPFRYDGSPTVGSDGKLGSHGTHVGGIAAGSRDGSPMHGVAYDAQIISADNGDPGP
EDGIILGNDGAVYKAGWDALIASGARIINNSWGIGITDRFDQGGRDPAFPHFTVQDAQLQ
FDQIKPLLGTKPGGAYEGAIAAARSGIVTIFAAGNDYNLNNPDAIAGLAYFVPDIAPNWL
TVAALQVNPDTASADPYTISTFSSRCGYTASFCVSAPGTRVYSAVIGGTRADDLTVGYAN
KSGTSMAAPHVAGSVAVLMERFPYMTGAQVASVLRTTATDMGAPGVDSLYGWGMINLGKA
IDGPSMLVTEQDIPAEFRIDGAYGSGQFVVDLPGIGAIIDAGKPTERVCSGIQCGLDVWR
NDIAGHGGLTKEGIGTLVLTGANTYSGPTLVNQGRLAINGSLASAVTVNDGGILGGNGRI
ASLTASRGGSVAPGNSIGTLQVAGDVTFQPGSNYSVEVSPTASDLIVADGKASIDGATVS
LSLENSPTLLTAGEVKSLLGRQFNILQAADGIDGRFAGVLPNYAFLGGDLAYSTSGIQLA
VERNDASFASLGLTPNQRSVASAAERLGAGNGLYEALLLSPSTAVAQQAFQQLSGEIHPA
IGTLLINDSRQLRDAVGDRLRQDALYDAGTPTDASSNAWFKVLGAWGKNDGGNDHASTTS
SIGGLLAGVDGLISDQTRLGFVTGYSDSSLSMGDGTHSSAKADSYHLGAYLGHEIDALRL
TVGAAHSWHRVDVKRDLQWGDVSGREKTKRDARTTQLFTEAAYRLNLQPVALEPFANLAY
VHLDSDSFHEKGDNAALHGGDDRRDAVLSTLGVRANHTLALSDRQQLQLSGSLGWQHNLS
STTSEQDLAFAGSSNTFAVQSVSMDRNAAVIGARAGLAVAKDVRLNLDYNGLIGSRDKTH
GVGLTLDWQF