Protein Info for Pf6N2E2_1228 in Pseudomonas fluorescens FW300-N2E2

Annotation: Lipase (EC 3.1.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 PF00353: HemolysinCabind" amino acids 367 to 397 (31 residues), 28.7 bits, see alignment (E = 5.1e-11) amino acids 391 to 415 (25 residues), 13.2 bits, see alignment (E = 3.6e-06) amino acids 493 to 526 (34 residues), 35.4 bits, see alignment (E = 4.3e-13) amino acids 510 to 544 (35 residues), 40.6 bits, see alignment (E = 9.9e-15) amino acids 520 to 545 (26 residues), 27.6 bits, see alignment (E = 1.1e-10)

Best Hits

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a2827)

Predicted SEED Role

"Lipase (EC 3.1.1.3)" (EC 3.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.3

Use Curated BLAST to search for 3.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWU7 at UniProt or InterPro

Protein Sequence (617 amino acids)

>Pf6N2E2_1228 Lipase (EC 3.1.1.3) (Pseudomonas fluorescens FW300-N2E2)
MGIYDYKNLGTAESKALVSDAMAIMLYSYHNLDNGFATGYQHNGFGAGLPATLVTALLGG
TDSQGVIPGVPWNPDSEKLALEAVQKAGWTPISAAQLGYAGKVDGRGTFFGEKAGYTTAQ
VEILGKYDSAGHLQEIGVSFRGTSGPRENLIGDSIGDAVNDLMAALGPKDYAKNYVGEAF
GNLLGDVAKFAQAHGLTGNDVLVSGHSLGGLAVNSLADLSTSQWSGFYSSANYIAYASPT
QSSTDKVLNIGYENDPVFRALDGSTFNLASVGVHDGQQESATNNIVSFNDHYASATWNVL
PFSILNIPTWISHLPTGYGDGMGRIMGSAFYDLTEKDSTIIVANLSDPARANTWVQDLNR
NAEPHTGSTFIIGSDSDDLIQGGQGNDYLEGRAGNDTFRDGGGYNVILGGQGSNSLQLQQ
SIQDFSVAHDGAGTLYVRDAHGAISITRDIGTLISKESGLFWGLLKDEVSHSVTANGLVA
GANLTAYASSIKGSVANDTLVAGAKGDWLFGQDGNDVLVGGAGNDTFVGGAGNDQMQAGA
GSDTFLFNGAFGQDRISGYDAGDKLVFLGVPGAGAGHDYSQYLSQVGNDTLFKVGDSSVT
LVGVGLGQVGGEGIVFA