Protein Info for Pf6N2E2_1207 in Pseudomonas fluorescens FW300-N2E2

Annotation: Epoxide hydrolase (EC 3.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 84 to 215 (132 residues), 72.4 bits, see alignment E=4.7e-24 PF12146: Hydrolase_4" amino acids 85 to 210 (126 residues), 29.7 bits, see alignment E=4.2e-11

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a2850)

Predicted SEED Role

"Epoxide hydrolase (EC 3.3.2.9)" (EC 3.3.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.3.2.9

Use Curated BLAST to search for 3.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZVN3 at UniProt or InterPro

Protein Sequence (357 amino acids)

>Pf6N2E2_1207 Epoxide hydrolase (EC 3.3.2.9) (Pseudomonas fluorescens FW300-N2E2)
MDTTLNHPSAARHGRLFTPALLVFAMMQLGALGFASSQANAADVPAGTIGAITPGSHTSF
GPLKHVKAGLLDVSYAEVGPADGPVVILLHGWPYDIHSYADVAPALAEKGYRVLIPYARG
YGDTRFLSAKTLRNGQPSALASDLIDFMDALKIKQAVLGGYDWGARTADIVAALWPERVK
ALVAVSGYLIGSQEAGKTPLPPAAELQWWYQFYFATERGRLGYEKNTHDFAKLIWKLASP
KWNFDDATYERSAAALQNPDHVAVSIFNYRWRLGVVKGEAKYDALEKKLATFPSISVPTI
TLEGDANGAPHPPVEAYAKRFTGQYEHRLIDGGVGHNLPQEAPRAFAQAVIDADHLQ