Protein Info for Pf6N2E2_1114 in Pseudomonas fluorescens FW300-N2E2

Annotation: Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 853 PF07992: Pyr_redox_2" amino acids 14 to 293 (280 residues), 179.3 bits, see alignment E=3.5e-56 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 14 to 807 (794 residues), 1060.2 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 155 to 224 (70 residues), 52.5 bits, see alignment E=1.7e-17 PF18267: Rubredoxin_C" amino acids 327 to 397 (71 residues), 40.9 bits, see alignment E=5e-14 PF04324: Fer2_BFD" amino acids 428 to 476 (49 residues), 38.8 bits, see alignment 2.9e-13 PF03460: NIR_SIR_ferr" amino acids 566 to 628 (63 residues), 40.9 bits, see alignment 4.1e-14 PF01077: NIR_SIR" amino acids 638 to 759 (122 residues), 79.7 bits, see alignment E=5.2e-26

Best Hits

Swiss-Prot: 63% identical to NIRB_KLEOX: Nitrite reductase [NAD(P)H] large subunit (nasB) from Klebsiella oxytoca

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 99% identity to pba:PSEBR_a2966)

MetaCyc: 58% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YYZ6 at UniProt or InterPro

Protein Sequence (853 amino acids)

>Pf6N2E2_1114 Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) (Pseudomonas fluorescens FW300-N2E2)
MNSNVASLNKLQTLIVIGNGMVGHHCVEQLIERGALNHYRVHVFSEEPMRAYDRVHLSEY
FSGRDAESLALGEASLYQTPGITLHLGVPVLEIDRDAHQVVTAHDRLHYDKLVLATGSYP
FVPPIEGAEGDSCLVYRTLEDLDAIRAAASNARRGVVVGGGLLGLEAANALKTLGLEAHV
VEFAPRLMPVQLDPQGGLALKARIEKLGVGVHLSKGTQSISAGEQYRYRMNFGDDDFLET
DLIVFSAGIRAQDALARQSDLQIGPRGGVVIDDHCQSSDPDIYAIGECAAWNGSIFGLVA
PGYQMARNVAARLCGESADAFTGADMSTKLKLLGVDVGSIGDAHGNTPGSRSFQFIDETS
ASYRRLVVDAAGKRVIGAVLVGDNSYYDTLLQYMQNGIALPKDAASLILPSSEGAPTLGP
AALPETATICSCHNVTKGSICSAIDGGCTDLGQLKCDTKAGTGCGGCAALVKQVFEHELT
ARGVSVDKSLCEHFAYTRQELYALVRVEGIISFEELLAKHGRGHTGCDLCKPAVGSILAS
CWNQPIMDPSLVPLQDTNDTFMANMQKNGTYSVVPRIAGGEITADKLIAIGVVAKKYDLY
TKITGGQRIDLFGAQLHQLPDIWAELIEAGFETGHAYGKSTRTVKSCVGSTWCRYGVQDS
VQMALTLEDRYKGLRSPHKLKFAVSGCTRECAEAQSKDVGVIATEKGWNLYVAGNGGMRP
RHAELFATDLDDATLIRYIDRFLMFYIRTADKLQRTSVWRESLEGGLDYLKEVIIDDSLG
LGAELEAQMQLVVDRYECEWANALKDPDKLKRFRTFVNDGRGDPDVHFVRERGQRRPVHA
HELHLIPVTEEVL