Protein Info for Pf6N2E2_1065 in Pseudomonas fluorescens FW300-N2E2

Annotation: Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 145 PF13412: HTH_24" amino acids 3 to 49 (47 residues), 39.3 bits, see alignment E=7.5e-14 PF13404: HTH_AsnC-type" amino acids 3 to 43 (41 residues), 41.5 bits, see alignment E=1.9e-14 PF09339: HTH_IclR" amino acids 10 to 49 (40 residues), 22.5 bits, see alignment E=1.5e-08 PF01037: AsnC_trans_reg" amino acids 68 to 134 (67 residues), 50.7 bits, see alignment E=2.8e-17

Best Hits

Swiss-Prot: 30% identical to REG7_PYRHO: HTH-type transcriptional regulator LrpA (lrpA) from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a3017)

Predicted SEED Role

"Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system" in subsystem Branched-Chain Amino Acid Biosynthesis or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWH4 at UniProt or InterPro

Protein Sequence (145 amino acids)

>Pf6N2E2_1065 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system (Pseudomonas fluorescens FW300-N2E2)
MTDDIDQILISALMEDSRRSLKALANLSGLSSPSVAERLRRLEERGVLRGYTVEVDPKCF
GYQLQAIVRIRPLPGQLQEVERQIQAIAEFTECDKVTGDDCFIARLHVRSMEQLDTLLDK
LNIHAETNTAIVKKTPVKRRLPPMA