Protein Info for Pf6N2E2_1023 in Pseudomonas fluorescens FW300-N2E2

Annotation: ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF12974: Phosphonate-bd" amino acids 64 to 194 (131 residues), 34.6 bits, see alignment E=3e-12 PF00497: SBP_bac_3" amino acids 66 to 225 (160 residues), 30.8 bits, see alignment E=4.3e-11 PF09084: NMT1" amino acids 67 to 257 (191 residues), 47.6 bits, see alignment E=4.2e-16

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 64% identity to avn:Avin_21640)

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWE4 at UniProt or InterPro

Protein Sequence (330 amino acids)

>Pf6N2E2_1023 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components (Pseudomonas fluorescens FW300-N2E2)
MNLLNRRDFLTASSFALGALGLSSLGLAPRVFAQGKQLADLTLGVATYRGQDSYFVEDAG
TGNTPYKVEYSEFAGGNLIVEALASGSLDVGGMSEIPPIFSIQSHRQPRLIAVLQSDVNN
QVFLIPKDSPIESIGQLRGKRVGYVRSTTSHYFLIKALKEQGLTMNDITAVALTPQDGFS
AFQSGQLDAWVIYGVFIQLAKFRSGARVLKTALGYLSGNYLIAARPAALEDPLRKQAIQD
YIQRQARTWEWINNNPEPWATKSAQLLGVDKAVFLDMYNNRSQPTKVVEVNDQAIASQQE
VADLFFAAGVLNERLDVSPLWDRNFRLLPL