Protein Info for Pf6N2E2_1002 in Pseudomonas fluorescens FW300-N2E2

Annotation: Myo-inositol 2-dehydrogenase (EC 1.1.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR04380: inositol 2-dehydrogenase" amino acids 1 to 329 (329 residues), 381.8 bits, see alignment E=1.1e-118 PF01408: GFO_IDH_MocA" amino acids 2 to 119 (118 residues), 83.4 bits, see alignment E=4.2e-27 PF03807: F420_oxidored" amino acids 3 to 82 (80 residues), 23.3 bits, see alignment E=1.6e-08 PF22725: GFO_IDH_MocA_C3" amino acids 128 to 248 (121 residues), 98.2 bits, see alignment E=7.3e-32 PF02894: GFO_IDH_MocA_C" amino acids 132 to 329 (198 residues), 98.8 bits, see alignment E=8e-32

Best Hits

KEGG orthology group: K00010, myo-inositol 2-dehydrogenase [EC: 1.1.1.18] (inferred from 70% identity to bam:Bamb_3338)

Predicted SEED Role

"Myo-inositol 2-dehydrogenase (EC 1.1.1.18)" in subsystem Inositol catabolism (EC 1.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.18

Use Curated BLAST to search for 1.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZUP4 at UniProt or InterPro

Protein Sequence (333 amino acids)

>Pf6N2E2_1002 Myo-inositol 2-dehydrogenase (EC 1.1.1.18) (Pseudomonas fluorescens FW300-N2E2)
MLRIAVLGAGRIGKIHAANVATNPRTKLVVIADPWKEGVNALAMQLGCEAAYDYASAIER
TDVDAVFIGTPTEFHIELMLQAVRLGKPVICEKPIDMDYERAKAAVDELERLNGRVMLAF
NRRFDPDYLRLRRAIDDGEIGVVRQVIITSRDPALAARAYLETSGGIFRDMTIHDFDMAR
SLLGEEPVEVFAVGSRLVDPTLESIPDYDSVMVLMRTASGRQCHINCCREAVYGFDQRLE
VSGSKGMLLNENHRTNSVRRYGAEATDVADPLLNFFLERHADSYKIELNSFLDAVENNSP
MPATPRDGLRALRLANCASESAETGKVVRVGDH