Protein Info for Pf1N1B4_992 in Pseudomonas fluorescens FW300-N1B4

Annotation: alginate o-acetyltransferase AlgF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 221 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF11182: AlgF" amino acids 22 to 199 (178 residues), 266.4 bits, see alignment E=4.7e-84

Best Hits

Swiss-Prot: 89% identical to ALGF_PSEFL: Alginate biosynthesis protein AlgF (algF) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 91% identity to pba:PSEBR_a4600)

MetaCyc: 63% identical to AlgF (Pseudomonas aeruginosa)
RXN-16462

Predicted SEED Role

"alginate o-acetyltransferase AlgF" in subsystem Alginate metabolism

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MYD4 at UniProt or InterPro

Protein Sequence (221 amino acids)

>Pf1N1B4_992 alginate o-acetyltransferase AlgF (Pseudomonas fluorescens FW300-N1B4)
MTFTTTPRRLAARSLKAVALVAGMSVLSMQAFAGDAALYGPTAPKGSSFVRVYNAANAEV
SATVGSTNLSEVAPLSSTDFSFMPGGDYSAKVGSQTLPVKLAGDHYYTLVNNASGAPQLI
EEPPFKNKQKSLVRVQNLSDKALTLKTADGKTEVVPSVAAKGRGEREINPVKVSLALYDG
ATKVGDVKPVALERGEAAVLYVTGSGSSLSPVWVKRPVSTR