Protein Info for Pf1N1B4_987 in Pseudomonas fluorescens FW300-N1B4

Annotation: Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF05048: NosD" amino acids 274 to 376 (103 residues), 32.1 bits, see alignment E=8.2e-12 amino acids 276 to 419 (144 residues), 49.7 bits, see alignment E=3.3e-17 PF13229: Beta_helix" amino acids 274 to 418 (145 residues), 53 bits, see alignment E=3.1e-18 TIGR03804: parallel beta-helix repeat" amino acids 381 to 419 (39 residues), 24.7 bits, see alignment 7e-10

Best Hits

Swiss-Prot: 85% identical to ALGG_PSEFL: Mannuronan C5-epimerase (algG) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 90% identity to pfo:Pfl01_0955)

MetaCyc: 67% identical to mannuronan C5 epimerase (Pseudomonas aeruginosa)
RXN-9839 [EC: 5.1.3.37]; TRANS-RXN-269 [EC: 5.1.3.37]

Predicted SEED Role

"Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-)" in subsystem Alginate metabolism (EC 5.1.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.- or 5.1.3.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161YZV5 at UniProt or InterPro

Protein Sequence (529 amino acids)

>Pf1N1B4_987 Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-) (Pseudomonas fluorescens FW300-N1B4)
MNSAKKGSISLLAGALLLASAAAFATVEPAQPVQAGKSTTMAKGLQQAKTYTVSSAPTAP
LDLAKPKLPDTSGYTAEAIAAKIVRTKPGKISVRRMMQENALKDFIGGDNKMAEWVVRQH
GIPQAIFVDDGYMNLKDLAKKVPKQYFSETSPGVFLAKLPIVVGEKGILEIDKQTQELRL
SQEAGSFLVNDGQLFVRDTKITGWSEKANGPAAFKSPKEFRPFLLSWGGTETYIANSKIA
SFGYANSKSYGVSISQYTPNMAKVLKRPEPSGWIVGSEFSDMWYGFYCYETRDFVVKGNT
YKDNIVYGIDPHDRSHGLIIADNTVYGTKKKHGIIISREVNDSFIFNNRSYDNKLSGLVI
DRNSVNNLIAYNEIYKNHTDGITLYESADNLLWGNKVISNKRHGIRIRNSVNIRLYENIS
MANGLTGVYGHIKDLTDTDRDIKLDPFDAQVSLIVVGGELAANGSGPLSIDSPLSVELYR
VSMLAPTKSSGISFSGILGERQDEILDLLVRQQKAVLIDPVERQTEMRD