Protein Info for Pf1N1B4_984 in Pseudomonas fluorescens FW300-N1B4

Annotation: Alginate biosynthesis protein Alg44

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 transmembrane" amino acids 159 to 181 (23 residues), see Phobius details amino acids 184 to 185 (2 residues), see Phobius details PF07238: PilZ" amino acids 16 to 116 (101 residues), 51 bits, see alignment E=1.6e-17 PF13437: HlyD_3" amino acids 262 to 358 (97 residues), 44.9 bits, see alignment E=1.7e-15

Best Hits

Swiss-Prot: 71% identical to ALG44_PSESM: Alginate biosynthesis protein Alg44 (alg44) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 91% identity to pfo:Pfl01_0958)

MetaCyc: 57% identical to mannuronosyl transferase regulatory subunit (Pseudomonas aeruginosa)
Alginate synthase. [EC: 2.4.1.33]

Predicted SEED Role

"Alginate biosynthesis protein Alg44"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MYA7 at UniProt or InterPro

Protein Sequence (389 amino acids)

>Pf1N1B4_984 Alginate biosynthesis protein Alg44 (Pseudomonas fluorescens FW300-N1B4)
MNTAVNSNVVHESEAQRQHARVKIPAKLRFFGPDRTPVEARVIDLSAGGLAFNAGQLPLK
VGDVHKARLQFVIDNLGLAMDVELQIRSVDRQTGRIGCQFQNLEPRDISTLRHLITSHLA
GDIVSVGEMLATLQRDNFTKARKTKDGGHGMTPLGRLKAVTFSAGIFVVGLVAFGFIFKS
VYGMYFVSHAQAGLVSVQGMNITMPRDGTVQSLIKADGVAAKGAPLATFSTSMLDVLKGH
LDEGQLQPSKVEELFGKQMTGTLTSPCDCTVAQQLVANGQYASKGDVIFQLVPRNTEANV
EARFSYRQFGDVRPGTTVSFQIAGEDQTRTGKIVSSTSLKSADLSSDIRVQIKPDEPLDS
AFAGRPVEVNSTRGPNLSWLINKAMAAGL