Protein Info for Pf1N1B4_966 in Pseudomonas fluorescens FW300-N1B4

Annotation: Butyryl-CoA dehydrogenase (EC 1.3.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 9 to 119 (111 residues), 123.5 bits, E=1.1e-39
• PF02770: Acyl-CoA_dh_M
  • amino acids 123 to 218 (96 residues), 98 bits, E=5.9e-32
• PF00441: Acyl-CoA_dh_1
  • amino acids 230 to 378 (149 residues), 159.4 bits, E=1.5e-50
• PF08028: Acyl-CoA_dh_2
  • amino acids 250 to 367 (118 residues), 61.1 bits, E=2.9e-20

Best Hits

Swiss-Prot: 56% identical to ACADL_PIG: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) from Sus scrofa

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a4643)

MetaCyc: 53% identical to long-chain-acyl-CoA dehydrogenase monomer (Homo sapiens)
LONG-CHAIN-ACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-21775 [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• oleate β-oxidation (30/35 steps found)
• fatty acid salvage (6/6 steps found)
• adipate degradation (5/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2

Sequence Analysis Tools

See A0A166MXT6 at UniProt or InterPro

Protein Sequence (378 amino acids)

>Pf1N1B4_966 Butyryl-CoA dehydrogenase (EC 1.3.99.2) (Pseudomonas fluorescens FW300-N1B4)
MIPRTLFSSEHELFRDSVRTFLEKEAVPFHGQWEKQGYIDRQLWNKAGEAGMLCSHLPEE
YGGLGADFLYSAVVIEEVGRLGLTGIGFSLHSDIVAPYILHYGSEALKHKYLPKLVSGEM
VTAIAMTEPGAGSDLQGVKTTAVLDGDEYVINGSKTFITNGFLADLVIVVAKTDPKAGAK
GTSLFLVEANTPGFAKGRRLEKVGMKAQDTSELFFQDVRVPKENLLGQAGMGFAYLMQEL
PQERLTVAVGGLASAEAALQWTLDYTRERKAFGKAIADFQNTRFKLAEMATEIQIGRVFV
DRCLELHLQGKLDVPTAAMAKYWGTDLQCKVLDECVQLHGGYGFMWEYPIARAWADARVQ
RIYAGTNEIMKEIIARSL